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Expanded breadth of virus neutralization after immunization with a multiclade envelope HIV vaccine candidate.

Publication ,  Journal Article
Chakrabarti, BK; Ling, X; Yang, Z-Y; Montefiori, DC; Panet, A; Kong, W-P; Welcher, B; Louder, MK; Mascola, JR; Nabel, GJ
Published in: Vaccine
May 16, 2005

Although the V3 loop of the human immunodeficiency virus type 1 (HIV-1) envelope (Env) effectively elicits potent neutralizing antibody responses, the specificity of the antibody response is often restricted to T cell line adapted (TCLA) strains and a small subset of primary isolates, limiting its utility for an AIDS vaccine. In this study, we have compared Env immunogens with substituted V3 regions to combinations of strains from different clades and evaluated their ability to expand the breadth of the neutralizing antibody response. When the V3 region from HIV BaL was substituted for HIV HXB2, an effective neutralizing antibody response against several clade B primary isolates was elicited, but it remained restricted to neutralization of most clade B isolates. In an attempt to expand this response further, a linear epitope recognized by the broadly neutralizing 2F5 antibody was inserted into V3. A V3 2F5 epitope was identified that bound to 2F5 and elicited a potent 2F5 antibody response as an immunogen, but the antisera neutralized only a lab-adapted strain and not primary isolates. In contrast, combinations of Envs from clades A, B, and C, elicited neutralizing antibodies to a more diverse group of primary HIV-1 isolates. These studies suggest that combinations of Env immunogens, despite the limited reactivity of the V3 from each component, can be used to expand the breadth of the neutralizing antibody response.

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Published In

Vaccine

DOI

ISSN

0264-410X

Publication Date

May 16, 2005

Volume

23

Issue

26

Start / End Page

3434 / 3445

Location

Netherlands

Related Subject Headings

  • Virology
  • Viral Envelope Proteins
  • Peptide Fragments
  • Neutralization Tests
  • Immunization
  • Humans
  • HIV-1
  • HIV Infections
  • HIV Antibodies
  • AIDS Vaccines
 

Citation

APA
Chicago
ICMJE
MLA
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Chakrabarti, B. K., Ling, X., Yang, Z.-Y., Montefiori, D. C., Panet, A., Kong, W.-P., … Nabel, G. J. (2005). Expanded breadth of virus neutralization after immunization with a multiclade envelope HIV vaccine candidate. Vaccine, 23(26), 3434–3445. https://doi.org/10.1016/j.vaccine.2005.01.099
Chakrabarti, Bimal K., Xu Ling, Zhi-Yong Yang, David C. Montefiori, Amos Panet, Wing-Pui Kong, Brent Welcher, Mark K. Louder, John R. Mascola, and Gary J. Nabel. “Expanded breadth of virus neutralization after immunization with a multiclade envelope HIV vaccine candidate.Vaccine 23, no. 26 (May 16, 2005): 3434–45. https://doi.org/10.1016/j.vaccine.2005.01.099.
Chakrabarti BK, Ling X, Yang Z-Y, Montefiori DC, Panet A, Kong W-P, et al. Expanded breadth of virus neutralization after immunization with a multiclade envelope HIV vaccine candidate. Vaccine. 2005 May 16;23(26):3434–45.
Chakrabarti, Bimal K., et al. “Expanded breadth of virus neutralization after immunization with a multiclade envelope HIV vaccine candidate.Vaccine, vol. 23, no. 26, May 2005, pp. 3434–45. Pubmed, doi:10.1016/j.vaccine.2005.01.099.
Chakrabarti BK, Ling X, Yang Z-Y, Montefiori DC, Panet A, Kong W-P, Welcher B, Louder MK, Mascola JR, Nabel GJ. Expanded breadth of virus neutralization after immunization with a multiclade envelope HIV vaccine candidate. Vaccine. 2005 May 16;23(26):3434–3445.
Journal cover image

Published In

Vaccine

DOI

ISSN

0264-410X

Publication Date

May 16, 2005

Volume

23

Issue

26

Start / End Page

3434 / 3445

Location

Netherlands

Related Subject Headings

  • Virology
  • Viral Envelope Proteins
  • Peptide Fragments
  • Neutralization Tests
  • Immunization
  • Humans
  • HIV-1
  • HIV Infections
  • HIV Antibodies
  • AIDS Vaccines