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Vaccination of macaques with SIV immunogens delivered by Venezuelan equine encephalitis virus replicon particle vectors followed by a mucosal challenge with SIVsmE660.

Publication ,  Journal Article
Johnston, RE; Johnson, PR; Connell, MJ; Montefiori, DC; West, A; Collier, ML; Cecil, C; Swanstrom, R; Frelinger, JA; Davis, NL
Published in: Vaccine
October 10, 2005

VEE replicon particles (VRP), non-propagating vaccine vectors derived from Venezuelan equine encephalitis virus (VEE), were engineered to express immunogens from the cloned isolate SIVsmH-4, combined in a vaccine cocktail and inoculated subcutaneously to immunize rhesus macaques. The virulent, uncloned challenge stock, SIVsmE660, represented a type of heterologous challenge and the intrarectal challenge modeled infection across a mucosal surface. Prechallenge neutralizing antibodies against SIVsmH-4 were induced in all vaccinates, and a prechallenge cellular immune response could be detected in one of six. Post-challenge, virus loads were reduced at the peak, at set point and at termination (41 weeks post-challenge), although these differences did not reach statistical significance. Significantly elevated levels of CD4+ T cells were observed post-challenge. A strong correlation was noted between a net increase in CD4+ T cell count and lowered virus load at set point.

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Published In

Vaccine

DOI

ISSN

0264-410X

Publication Date

October 10, 2005

Volume

23

Issue

42

Start / End Page

4969 / 4979

Location

Netherlands

Related Subject Headings

  • Virology
  • Viral Vaccines
  • Viral Load
  • Vaccines, Synthetic
  • T-Lymphocytes, Cytotoxic
  • Simian Acquired Immunodeficiency Syndrome
  • SAIDS Vaccines
  • Replicon
  • Neutralization Tests
  • Macaca mulatta
 

Citation

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Chicago
ICMJE
MLA
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Johnston, R. E., Johnson, P. R., Connell, M. J., Montefiori, D. C., West, A., Collier, M. L., … Davis, N. L. (2005). Vaccination of macaques with SIV immunogens delivered by Venezuelan equine encephalitis virus replicon particle vectors followed by a mucosal challenge with SIVsmE660. Vaccine, 23(42), 4969–4979. https://doi.org/10.1016/j.vaccine.2005.05.034
Johnston, Robert E., Philip R. Johnson, Mary J. Connell, David C. Montefiori, Ande West, Martha L. Collier, Chad Cecil, Ronald Swanstrom, Jeffrey A. Frelinger, and Nancy L. Davis. “Vaccination of macaques with SIV immunogens delivered by Venezuelan equine encephalitis virus replicon particle vectors followed by a mucosal challenge with SIVsmE660.Vaccine 23, no. 42 (October 10, 2005): 4969–79. https://doi.org/10.1016/j.vaccine.2005.05.034.
Johnston RE, Johnson PR, Connell MJ, Montefiori DC, West A, Collier ML, et al. Vaccination of macaques with SIV immunogens delivered by Venezuelan equine encephalitis virus replicon particle vectors followed by a mucosal challenge with SIVsmE660. Vaccine. 2005 Oct 10;23(42):4969–79.
Johnston, Robert E., et al. “Vaccination of macaques with SIV immunogens delivered by Venezuelan equine encephalitis virus replicon particle vectors followed by a mucosal challenge with SIVsmE660.Vaccine, vol. 23, no. 42, Oct. 2005, pp. 4969–79. Pubmed, doi:10.1016/j.vaccine.2005.05.034.
Johnston RE, Johnson PR, Connell MJ, Montefiori DC, West A, Collier ML, Cecil C, Swanstrom R, Frelinger JA, Davis NL. Vaccination of macaques with SIV immunogens delivered by Venezuelan equine encephalitis virus replicon particle vectors followed by a mucosal challenge with SIVsmE660. Vaccine. 2005 Oct 10;23(42):4969–4979.
Journal cover image

Published In

Vaccine

DOI

ISSN

0264-410X

Publication Date

October 10, 2005

Volume

23

Issue

42

Start / End Page

4969 / 4979

Location

Netherlands

Related Subject Headings

  • Virology
  • Viral Vaccines
  • Viral Load
  • Vaccines, Synthetic
  • T-Lymphocytes, Cytotoxic
  • Simian Acquired Immunodeficiency Syndrome
  • SAIDS Vaccines
  • Replicon
  • Neutralization Tests
  • Macaca mulatta