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Evidence for epithelial-mesenchymal transitions in adult liver cells.

Publication ,  Journal Article
Sicklick, JK; Choi, SS; Bustamante, M; McCall, SJ; Pérez, EH; Huang, J; Li, Y-X; Rojkind, M; Diehl, AM
Published in: Am J Physiol Gastrointest Liver Physiol
October 2006

Both myofibroblastic hepatic stellate cells (HSC) and hepatic epithelial progenitors accumulate in damaged livers. In some injured organs, the ability to distinguish between fibroblastic and epithelial cells is sometimes difficult because cells undergo epithelial-mesenchymal transitions (EMT). During EMT, cells coexpress epithelial and mesenchymal cell markers. To determine whether EMT occurs in adult liver cells, we analyzed the expression profile of primary HSC, two HSC lines, and hepatic epithelial progenitors. As expected, all HSC expressed HSC markers. Surprisingly, these markers were also expressed by epithelial progenitors. In addition, one HSC line expressed typical epithelial progenitor mRNAs, and these epithelial markers were inducible in the second HSC line. In normal and damaged livers, small ductular-type cells stained positive for an HSC marker. In conclusion, HSC and hepatic epithelial progenitors both coexpress epithelial and mesenchymal markers, providing evidence that EMT occurs in adult liver cells.

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Published In

Am J Physiol Gastrointest Liver Physiol

DOI

ISSN

0193-1857

Publication Date

October 2006

Volume

291

Issue

4

Start / End Page

G575 / G583

Location

United States

Related Subject Headings

  • Rats
  • Mice, Inbred C57BL
  • Mice
  • Mesenchymal Stem Cells
  • Male
  • Liver
  • Humans
  • Gene Expression Regulation, Developmental
  • Gastroenterology & Hepatology
  • Epithelial Cells
 

Citation

APA
Chicago
ICMJE
MLA
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Sicklick, J. K., Choi, S. S., Bustamante, M., McCall, S. J., Pérez, E. H., Huang, J., … Diehl, A. M. (2006). Evidence for epithelial-mesenchymal transitions in adult liver cells. Am J Physiol Gastrointest Liver Physiol, 291(4), G575–G583. https://doi.org/10.1152/ajpgi.00102.2006
Sicklick, Jason K., Steve S. Choi, Marcia Bustamante, Shannon J. McCall, Elizabeth Hernández Pérez, Jiawen Huang, Yin-Xiong Li, Marcos Rojkind, and Anna Mae Diehl. “Evidence for epithelial-mesenchymal transitions in adult liver cells.Am J Physiol Gastrointest Liver Physiol 291, no. 4 (October 2006): G575–83. https://doi.org/10.1152/ajpgi.00102.2006.
Sicklick JK, Choi SS, Bustamante M, McCall SJ, Pérez EH, Huang J, et al. Evidence for epithelial-mesenchymal transitions in adult liver cells. Am J Physiol Gastrointest Liver Physiol. 2006 Oct;291(4):G575–83.
Sicklick, Jason K., et al. “Evidence for epithelial-mesenchymal transitions in adult liver cells.Am J Physiol Gastrointest Liver Physiol, vol. 291, no. 4, Oct. 2006, pp. G575–83. Pubmed, doi:10.1152/ajpgi.00102.2006.
Sicklick JK, Choi SS, Bustamante M, McCall SJ, Pérez EH, Huang J, Li Y-X, Rojkind M, Diehl AM. Evidence for epithelial-mesenchymal transitions in adult liver cells. Am J Physiol Gastrointest Liver Physiol. 2006 Oct;291(4):G575–G583.

Published In

Am J Physiol Gastrointest Liver Physiol

DOI

ISSN

0193-1857

Publication Date

October 2006

Volume

291

Issue

4

Start / End Page

G575 / G583

Location

United States

Related Subject Headings

  • Rats
  • Mice, Inbred C57BL
  • Mice
  • Mesenchymal Stem Cells
  • Male
  • Liver
  • Humans
  • Gene Expression Regulation, Developmental
  • Gastroenterology & Hepatology
  • Epithelial Cells