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Neonatal Escherichia coli infection alters glial, cytokine, and neuronal gene expression in response to acute amphetamine in adolescent rats.

Publication ,  Journal Article
Bland, ST; Beckley, JT; Watkins, LR; Maier, SF; Bilbo, SD
Published in: Neuroscience letters
April 2010

Neonatal bacterial infection in rats alters the responses to a variety of subsequent challenges later in life. Here we explored the effects of neonatal bacterial infection on a subsequent drug challenge during adolescence, using administration of the psychostimulant amphetamine. Male rat pups were injected on postnatal day 4 (P4) with live Escherichia coli (E. coli) or PBS vehicle, and then received amphetamine (15mg/kg) or saline on P40. Quantitative RT-PCR was performed on micropunches taken from medial prefrontal cortex, nucleus accumbens, and the CA1 subfield of the hippocampus. mRNA for glial and neuronal activation markers as well as pro-inflammatory and anti-inflammatory cytokines were assessed. Amphetamine produced brain region specific increases in many of these genes in PBS controls, while these effects were blunted or absent in neonatal E. coli treated rats. In contrast to the potentiating effect of neonatal E. coli on glial and cytokine responses to an immune challenge previously observed, neonatal E. coli infection attenuates glial and cytokine responses to an amphetamine challenge.

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Published In

Neuroscience letters

DOI

EISSN

1872-7972

ISSN

0304-3940

Publication Date

April 2010

Volume

474

Issue

1

Start / End Page

52 / 57

Related Subject Headings

  • Rats
  • RNA, Messenger
  • Neurons
  • Neuroglia
  • Nerve Tissue Proteins
  • Membrane Proteins
  • Male
  • Glial Fibrillary Acidic Protein
  • Gene Expression
  • Escherichia coli Infections
 

Citation

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Bland, S. T., Beckley, J. T., Watkins, L. R., Maier, S. F., & Bilbo, S. D. (2010). Neonatal Escherichia coli infection alters glial, cytokine, and neuronal gene expression in response to acute amphetamine in adolescent rats. Neuroscience Letters, 474(1), 52–57. https://doi.org/10.1016/j.neulet.2010.03.006
Bland, Sondra T., Jacob T. Beckley, Linda R. Watkins, Steven F. Maier, and Staci D. Bilbo. “Neonatal Escherichia coli infection alters glial, cytokine, and neuronal gene expression in response to acute amphetamine in adolescent rats.Neuroscience Letters 474, no. 1 (April 2010): 52–57. https://doi.org/10.1016/j.neulet.2010.03.006.
Bland ST, Beckley JT, Watkins LR, Maier SF, Bilbo SD. Neonatal Escherichia coli infection alters glial, cytokine, and neuronal gene expression in response to acute amphetamine in adolescent rats. Neuroscience letters. 2010 Apr;474(1):52–7.
Bland, Sondra T., et al. “Neonatal Escherichia coli infection alters glial, cytokine, and neuronal gene expression in response to acute amphetamine in adolescent rats.Neuroscience Letters, vol. 474, no. 1, Apr. 2010, pp. 52–57. Epmc, doi:10.1016/j.neulet.2010.03.006.
Bland ST, Beckley JT, Watkins LR, Maier SF, Bilbo SD. Neonatal Escherichia coli infection alters glial, cytokine, and neuronal gene expression in response to acute amphetamine in adolescent rats. Neuroscience letters. 2010 Apr;474(1):52–57.
Journal cover image

Published In

Neuroscience letters

DOI

EISSN

1872-7972

ISSN

0304-3940

Publication Date

April 2010

Volume

474

Issue

1

Start / End Page

52 / 57

Related Subject Headings

  • Rats
  • RNA, Messenger
  • Neurons
  • Neuroglia
  • Nerve Tissue Proteins
  • Membrane Proteins
  • Male
  • Glial Fibrillary Acidic Protein
  • Gene Expression
  • Escherichia coli Infections