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The corneal epithelium after optisol-GS storage.

Publication ,  Journal Article
Means, TL; Geroski, DH; L'Hernault, N; Grossniklaus, HE; Kim, T; Edelhauser, HF
Published in: Cornea
November 1996

The objective of this study was to evaluate the epithelium of human corneas stored in Optisol-GS (Chiron Intraoptics, Irvine, CA) for extended periods (2-34 days). Human corneas stored in Optisol-GS (n = 64) were obtained from the Georgia Eye Bank. Corneal epithelial viability was assessed by using the Calcein-AM (Molecular Probes, Inc., Eugene, OR) ethidium homodimer stain, a fluorescent assay used to distinguish live from dead cells. Scanning and transmission electron microscopy was used to evaluate epithelial ultrastructure. The results showed that corneas stored up to 6 days in Optisol-GS had minimal damage of the epithelium. Calcein-AM ethidium homodimer staining showed 20-25% epithelial damage. Corneas stored 7-10 days had a further increase in epithelial damage (30-35%). Corneas stored for 11-15 days had marked increases in epithelial damage (40-50%), and corneas stored 16-34 days showed significant epithelial damage (60-70%). The data show that corneas stored in Optisol-GS are able to maintain the epithelium up to 6 days. A gradual decrease in epithelial viability and loss of epithelial cells occurs in corneas stored 6-10 days. Corneas stored for > 10 days have a marked loss of epithelial cells with extensive epithelial damage.

Duke Scholars

Published In

Cornea

ISSN

0277-3740

Publication Date

November 1996

Volume

15

Issue

6

Start / End Page

599 / 605

Location

United States

Related Subject Headings

  • Time Factors
  • Organ Preservation
  • Ophthalmology & Optometry
  • Middle Aged
  • Microscopy, Electron, Scanning
  • Humans
  • Gentamicins
  • Fluorescent Dyes
  • Fluoresceins
  • Epithelium
 

Citation

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ICMJE
MLA
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Means, T. L., Geroski, D. H., L’Hernault, N., Grossniklaus, H. E., Kim, T., & Edelhauser, H. F. (1996). The corneal epithelium after optisol-GS storage. Cornea, 15(6), 599–605.
Means, T. L., D. H. Geroski, N. L’Hernault, H. E. Grossniklaus, T. Kim, and H. F. Edelhauser. “The corneal epithelium after optisol-GS storage.Cornea 15, no. 6 (November 1996): 599–605.
Means TL, Geroski DH, L’Hernault N, Grossniklaus HE, Kim T, Edelhauser HF. The corneal epithelium after optisol-GS storage. Cornea. 1996 Nov;15(6):599–605.
Means, T. L., et al. “The corneal epithelium after optisol-GS storage.Cornea, vol. 15, no. 6, Nov. 1996, pp. 599–605.
Means TL, Geroski DH, L’Hernault N, Grossniklaus HE, Kim T, Edelhauser HF. The corneal epithelium after optisol-GS storage. Cornea. 1996 Nov;15(6):599–605.

Published In

Cornea

ISSN

0277-3740

Publication Date

November 1996

Volume

15

Issue

6

Start / End Page

599 / 605

Location

United States

Related Subject Headings

  • Time Factors
  • Organ Preservation
  • Ophthalmology & Optometry
  • Middle Aged
  • Microscopy, Electron, Scanning
  • Humans
  • Gentamicins
  • Fluorescent Dyes
  • Fluoresceins
  • Epithelium