Accentuation of differentially expressed proteins using phage technology.
Publication
, Journal Article
Suber, RL; Flanders, VL; Campa, MJ; Patz, EF
Published in: Anal Biochem
October 15, 2004
Protein profiling is frequently used to elucidate disease-specific or differentially expressed proteins. While recent developments have resulted in improved differential profiling, alternative expression platforms that complement existing techniques are continually being explored. We developed a novel method utilizing the amplification and selection capabilities of random peptide-expressing M13 bacteriophage to accentuate differentially expressed proteins in biologic specimens. While the current study used this method to demonstrate differentially expressed proteins in lung cancer tissue in comparison to normal lung tissue, this approach is applicable to a wide range of sample types.
Duke Scholars
Published In
Anal Biochem
DOI
ISSN
0003-2697
Publication Date
October 15, 2004
Volume
333
Issue
2
Start / End Page
351 / 357
Location
United States
Related Subject Headings
- Tumor Cells, Cultured
- Proteomics
- Proteins
- Lung Neoplasms
- Lung
- Humans
- Gene Expression Regulation, Neoplastic
- Gene Expression Profiling
- Cells, Cultured
- Biochemistry & Molecular Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Suber, R. L., Flanders, V. L., Campa, M. J., & Patz, E. F. (2004). Accentuation of differentially expressed proteins using phage technology. Anal Biochem, 333(2), 351–357. https://doi.org/10.1016/j.ab.2004.06.029
Suber, R Lee, Vincent L. Flanders, Michael J. Campa, and Edward F. Patz. “Accentuation of differentially expressed proteins using phage technology.” Anal Biochem 333, no. 2 (October 15, 2004): 351–57. https://doi.org/10.1016/j.ab.2004.06.029.
Suber RL, Flanders VL, Campa MJ, Patz EF. Accentuation of differentially expressed proteins using phage technology. Anal Biochem. 2004 Oct 15;333(2):351–7.
Suber, R. Lee, et al. “Accentuation of differentially expressed proteins using phage technology.” Anal Biochem, vol. 333, no. 2, Oct. 2004, pp. 351–57. Pubmed, doi:10.1016/j.ab.2004.06.029.
Suber RL, Flanders VL, Campa MJ, Patz EF. Accentuation of differentially expressed proteins using phage technology. Anal Biochem. 2004 Oct 15;333(2):351–357.
Published In
Anal Biochem
DOI
ISSN
0003-2697
Publication Date
October 15, 2004
Volume
333
Issue
2
Start / End Page
351 / 357
Location
United States
Related Subject Headings
- Tumor Cells, Cultured
- Proteomics
- Proteins
- Lung Neoplasms
- Lung
- Humans
- Gene Expression Regulation, Neoplastic
- Gene Expression Profiling
- Cells, Cultured
- Biochemistry & Molecular Biology