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(1)H MRS detection of glycine residue of reduced glutathione in vivo.

Publication ,  Journal Article
Kaiser, LG; Marjańska, M; Matson, GB; Iltis, I; Bush, SD; Soher, BJ; Mueller, S; Young, K
Published in: J Magn Reson
February 2010

Glutathione (GSH) is a powerful antioxidant found inside different kinds of cells, including those of the central nervous system. Detection of GSH in the human brain using (1)H MR spectroscopy is hindered by low concentration and spectral overlap with other metabolites. Previous MRS methods focused mainly on the detection of the cysteine residue (GSH-Cys) via editing schemes. This study focuses on the detection of the glycine residue (GSH-Gly), which is overlapped by glutamate and glutamine (Glx) under physiological pH and temperature. The first goal of the study was to obtain the spectral parameters for characterization of the GSH-Gly signal under physiological conditions. The second goal was to investigate a new method of separating GSH-Gly from Glx in vivo. The characterization of the signal was carried out by utilization of numerical simulations as well as experiments over a wide range of magnetic fields (4.0-14T). The proposed separation scheme utilizes J-difference editing to quantify the Glx contribution to separate it from the GSH-Gly signal. The presented method retains 100% of the GSH-Gly signal. The overall increase in signal to noise ratio of the targeted resonance is calculated to yield a significant SNR improvement compared to previously used methods that target GSH-Cys residue. This allows shorter acquisition times for in vivo human clinical studies.

Duke Scholars

Published In

J Magn Reson

DOI

EISSN

1096-0856

Publication Date

February 2010

Volume

202

Issue

2

Start / End Page

259 / 266

Location

United States

Related Subject Headings

  • Temperature
  • Nuclear Magnetic Resonance, Biomolecular
  • Humans
  • Glycine
  • Glutathione
  • Electromagnetic Fields
  • Data Interpretation, Statistical
  • Cysteine
  • Computer Simulation
  • Biophysics
 

Citation

APA
Chicago
ICMJE
MLA
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Kaiser, L. G., Marjańska, M., Matson, G. B., Iltis, I., Bush, S. D., Soher, B. J., … Young, K. (2010). (1)H MRS detection of glycine residue of reduced glutathione in vivo. J Magn Reson, 202(2), 259–266. https://doi.org/10.1016/j.jmr.2009.11.013
Kaiser, Lana G., Małgorzata Marjańska, Gerald B. Matson, Isabelle Iltis, Seth D. Bush, Brian J. Soher, Susanne Mueller, and Karl Young. “(1)H MRS detection of glycine residue of reduced glutathione in vivo.J Magn Reson 202, no. 2 (February 2010): 259–66. https://doi.org/10.1016/j.jmr.2009.11.013.
Kaiser LG, Marjańska M, Matson GB, Iltis I, Bush SD, Soher BJ, et al. (1)H MRS detection of glycine residue of reduced glutathione in vivo. J Magn Reson. 2010 Feb;202(2):259–66.
Kaiser, Lana G., et al. “(1)H MRS detection of glycine residue of reduced glutathione in vivo.J Magn Reson, vol. 202, no. 2, Feb. 2010, pp. 259–66. Pubmed, doi:10.1016/j.jmr.2009.11.013.
Kaiser LG, Marjańska M, Matson GB, Iltis I, Bush SD, Soher BJ, Mueller S, Young K. (1)H MRS detection of glycine residue of reduced glutathione in vivo. J Magn Reson. 2010 Feb;202(2):259–266.
Journal cover image

Published In

J Magn Reson

DOI

EISSN

1096-0856

Publication Date

February 2010

Volume

202

Issue

2

Start / End Page

259 / 266

Location

United States

Related Subject Headings

  • Temperature
  • Nuclear Magnetic Resonance, Biomolecular
  • Humans
  • Glycine
  • Glutathione
  • Electromagnetic Fields
  • Data Interpretation, Statistical
  • Cysteine
  • Computer Simulation
  • Biophysics