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Quantitation of proton NMR spectra of the human brain using tissue water as an internal concentration reference.

Publication ,  Journal Article
Barker, PB; Soher, BJ; Blackband, SJ; Chatham, JC; Mathews, VP; Bryan, RN
Published in: NMR Biomed
1993

The use of cerebral water as an internal intensity standard for the quantitation of spatially localized proton spectra of the human brain is investigated. The method is validated on standard samples of N-acetyl aspartate (NAA) and lactate, and possible sources of error are discussed. Using the STEAM pulse sequence, concentrations of choline, creatine and NAA in frontal lobe white matter are found to be 1.9 +/- 0.5, 10.6 +/- 1.3 and 16.6 +/- 2.3 mumol/g wet wt, respectively, in 10 normal volunteers. In the thalamus, the concentrations are 2.0 +/- 0.4, 11.6 +/- 2.0 and 17.2 +/- 1.3 mumol/g wet wt, respectively. Choline and creatine concentrations are in good agreement with conventional biochemical values: NAA concentrations are found to be three-fold higher, suggesting overlap of the NAA signal with other compounds. Quantitation relative to tissue water is a convenient and rapid means of quantitating proton spectra of the human brain.

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Published In

NMR Biomed

DOI

ISSN

0952-3480

Publication Date

1993

Volume

6

Issue

1

Start / End Page

89 / 94

Location

England

Related Subject Headings

  • Water
  • Reproducibility of Results
  • Reference Standards
  • Protons
  • Nuclear Medicine & Medical Imaging
  • Middle Aged
  • Male
  • Magnetic Resonance Spectroscopy
  • Humans
  • Female
 

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Barker, P. B., Soher, B. J., Blackband, S. J., Chatham, J. C., Mathews, V. P., & Bryan, R. N. (1993). Quantitation of proton NMR spectra of the human brain using tissue water as an internal concentration reference. NMR Biomed, 6(1), 89–94. https://doi.org/10.1002/nbm.1940060114
Barker, P. B., B. J. Soher, S. J. Blackband, J. C. Chatham, V. P. Mathews, and R. N. Bryan. “Quantitation of proton NMR spectra of the human brain using tissue water as an internal concentration reference.NMR Biomed 6, no. 1 (1993): 89–94. https://doi.org/10.1002/nbm.1940060114.
Barker PB, Soher BJ, Blackband SJ, Chatham JC, Mathews VP, Bryan RN. Quantitation of proton NMR spectra of the human brain using tissue water as an internal concentration reference. NMR Biomed. 1993;6(1):89–94.
Barker, P. B., et al. “Quantitation of proton NMR spectra of the human brain using tissue water as an internal concentration reference.NMR Biomed, vol. 6, no. 1, 1993, pp. 89–94. Pubmed, doi:10.1002/nbm.1940060114.
Barker PB, Soher BJ, Blackband SJ, Chatham JC, Mathews VP, Bryan RN. Quantitation of proton NMR spectra of the human brain using tissue water as an internal concentration reference. NMR Biomed. 1993;6(1):89–94.
Journal cover image

Published In

NMR Biomed

DOI

ISSN

0952-3480

Publication Date

1993

Volume

6

Issue

1

Start / End Page

89 / 94

Location

England

Related Subject Headings

  • Water
  • Reproducibility of Results
  • Reference Standards
  • Protons
  • Nuclear Medicine & Medical Imaging
  • Middle Aged
  • Male
  • Magnetic Resonance Spectroscopy
  • Humans
  • Female