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Quantitative proton spectroscopy and histology of a canine brain tumor model.

Publication ,  Journal Article
Barker, PB; Blackband, SJ; Chatham, JC; Soher, BJ; Samphilipo, MA; Magee, CA; Hilton, JD; Strandberg, JD; Anderson, JH
Published in: Magn Reson Med
October 1993

Quantitative, single voxel proton nuclear magnetic resonance (NMR) spectroscopy and histological analysis was performed in eight dogs implanted with the transplantable canine glioma model of Wodinsky (Proc. Am. Assoc. Cancer Res. 10, 99 (1969)). Signals from choline, creatine, N-Acetyl Aspartate (NAA) and lactate were converted to molar concentration units and correlated with the quantitative analysis of histologically determined tissue types within the localized volume selected for NMR spectroscopy. In general, compared with normal brain, the lesions were associated with reductions in all metabolite concentrations, with the exception of lactate, which was increased. NAA and creatine decreases were most significantly correlated with the total lesion volume (P < 0.01), suggesting that these compounds are present in normal brain only. Changes in choline levels did not correlate strongly with any particular tissue type. Lactate was found to increase with increasing total lesion volume (P < 0.01), but not with increasing percent tumor, suggesting that it accumulates in abnormal tissue other than the tumor. The spectra reported were similar to those observed in human glioblastomas, with the exception that elevations of choline were not observed. The transplantable canine gliosarcoma system appears to be a suitable tumor model for evaluation by clinical radiological techniques such as magnetic resonance imaging (MRI) and proton NMR spectroscopy.

Duke Scholars

Published In

Magn Reson Med

DOI

ISSN

0740-3194

Publication Date

October 1993

Volume

30

Issue

4

Start / End Page

458 / 464

Location

United States

Related Subject Headings

  • Nuclear Medicine & Medical Imaging
  • Neoplasm Transplantation
  • Magnetic Resonance Spectroscopy
  • Lactic Acid
  • Lactates
  • Glioma
  • Dogs
  • Creatine
  • Choline
  • Brain Neoplasms
 

Citation

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Barker, P. B., Blackband, S. J., Chatham, J. C., Soher, B. J., Samphilipo, M. A., Magee, C. A., … Anderson, J. H. (1993). Quantitative proton spectroscopy and histology of a canine brain tumor model. Magn Reson Med, 30(4), 458–464. https://doi.org/10.1002/mrm.1910300408
Barker, P. B., S. J. Blackband, J. C. Chatham, B. J. Soher, M. A. Samphilipo, C. A. Magee, J. D. Hilton, J. D. Strandberg, and J. H. Anderson. “Quantitative proton spectroscopy and histology of a canine brain tumor model.Magn Reson Med 30, no. 4 (October 1993): 458–64. https://doi.org/10.1002/mrm.1910300408.
Barker PB, Blackband SJ, Chatham JC, Soher BJ, Samphilipo MA, Magee CA, et al. Quantitative proton spectroscopy and histology of a canine brain tumor model. Magn Reson Med. 1993 Oct;30(4):458–64.
Barker, P. B., et al. “Quantitative proton spectroscopy and histology of a canine brain tumor model.Magn Reson Med, vol. 30, no. 4, Oct. 1993, pp. 458–64. Pubmed, doi:10.1002/mrm.1910300408.
Barker PB, Blackband SJ, Chatham JC, Soher BJ, Samphilipo MA, Magee CA, Hilton JD, Strandberg JD, Anderson JH. Quantitative proton spectroscopy and histology of a canine brain tumor model. Magn Reson Med. 1993 Oct;30(4):458–464.
Journal cover image

Published In

Magn Reson Med

DOI

ISSN

0740-3194

Publication Date

October 1993

Volume

30

Issue

4

Start / End Page

458 / 464

Location

United States

Related Subject Headings

  • Nuclear Medicine & Medical Imaging
  • Neoplasm Transplantation
  • Magnetic Resonance Spectroscopy
  • Lactic Acid
  • Lactates
  • Glioma
  • Dogs
  • Creatine
  • Choline
  • Brain Neoplasms