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Quantitative proton spectroscopy of canine brain: in vivo and in vitro correlations.

Publication ,  Journal Article
Barker, PB; Breiter, SN; Soher, BJ; Chatham, JC; Forder, JR; Samphilipo, MA; Magee, CA; Anderson, JH
Published in: Magn Reson Med
August 1994

Quantitative, single-voxel proton NMR spectroscopy of normal brain was performed in five adult beagle dogs using the cerebral water signal as an internal intensity reference. The same brain regions were then rapidly isolated and frozen using a pneumatic biopsy drill, perchloric acid extracted, and analyzed by biochemical assay and high-resolution NMR spectroscopy. The concentrations of the major resonances in the in vivo and in vitro spectra were compared, and good agreement was found between the different measurements. The in vivo spectra contained three peaks at 3.21, 3.04, and 2.02 ppm, which are usually assigned to trimethylamines (TMA), creatines, and N-acetyl derivatives (NAc), which corresponded to be the following metabolite concentration values: 1.7 +/- 0.6, 7.7 +/- 2.1, and 10.9 +/- 2.7 mumol/g wet weight respectively. In vitro, the following metabolite concentrations were measured: glycerophosphocholine (GPC) 1.3 +/- 0.2, phosphocholine (PC) 0.5 +/- 0.1, phosphocreatine (PCr) 2.6 +/- 0.4, creatine (Cr) 5.9 +/- 1.4, and N-Acetyl aspartate (NAA) 8.9 +/- 1.8 mumol/g wet weight. Therefore, the 3.21 ppm resonance observed in the in vivo spectrum is predominantly GPC and PC in a ratio of 2.6:1, the 3.04 ppm resonance is Cr and PCr in a ratio of 2.3:1, and the 2.02 ppm resonance is predominantly (approximately 80%) NAA with small contributions from N-acetylaspartylglutamate (NAAG) and glutamate. The data presented here validate the technique of water referencing as a simple and convenient means of quantitating single-voxel in vivo proton NMR spectra of the brain.

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Published In

Magn Reson Med

DOI

ISSN

0740-3194

Publication Date

August 1994

Volume

32

Issue

2

Start / End Page

157 / 163

Location

United States

Related Subject Headings

  • Phosphorylcholine
  • Phosphocreatine
  • Nuclear Medicine & Medical Imaging
  • Methylamines
  • Magnetic Resonance Spectroscopy
  • In Vitro Techniques
  • Glycerylphosphorylcholine
  • Dogs
  • Brain Chemistry
  • Aspartic Acid
 

Citation

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Barker, P. B., Breiter, S. N., Soher, B. J., Chatham, J. C., Forder, J. R., Samphilipo, M. A., … Anderson, J. H. (1994). Quantitative proton spectroscopy of canine brain: in vivo and in vitro correlations. Magn Reson Med, 32(2), 157–163. https://doi.org/10.1002/mrm.1910320202
Barker, P. B., S. N. Breiter, B. J. Soher, J. C. Chatham, J. R. Forder, M. A. Samphilipo, C. A. Magee, and J. H. Anderson. “Quantitative proton spectroscopy of canine brain: in vivo and in vitro correlations.Magn Reson Med 32, no. 2 (August 1994): 157–63. https://doi.org/10.1002/mrm.1910320202.
Barker PB, Breiter SN, Soher BJ, Chatham JC, Forder JR, Samphilipo MA, et al. Quantitative proton spectroscopy of canine brain: in vivo and in vitro correlations. Magn Reson Med. 1994 Aug;32(2):157–63.
Barker, P. B., et al. “Quantitative proton spectroscopy of canine brain: in vivo and in vitro correlations.Magn Reson Med, vol. 32, no. 2, Aug. 1994, pp. 157–63. Pubmed, doi:10.1002/mrm.1910320202.
Barker PB, Breiter SN, Soher BJ, Chatham JC, Forder JR, Samphilipo MA, Magee CA, Anderson JH. Quantitative proton spectroscopy of canine brain: in vivo and in vitro correlations. Magn Reson Med. 1994 Aug;32(2):157–163.
Journal cover image

Published In

Magn Reson Med

DOI

ISSN

0740-3194

Publication Date

August 1994

Volume

32

Issue

2

Start / End Page

157 / 163

Location

United States

Related Subject Headings

  • Phosphorylcholine
  • Phosphocreatine
  • Nuclear Medicine & Medical Imaging
  • Methylamines
  • Magnetic Resonance Spectroscopy
  • In Vitro Techniques
  • Glycerylphosphorylcholine
  • Dogs
  • Brain Chemistry
  • Aspartic Acid