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Proteasome inhibition by chronic oxidative stress in human trabecular meshwork cells.

Publication ,  Journal Article
Caballero, M; Liton, PB; Epstein, DL; Gonzalez, P
Published in: Biochem Biophys Res Commun
August 22, 2003

The pathophysiologic mechanisms leading to the malfunction of the trabecular meshwork (TM)-Schlemm's canal (SC) outflow pathway in glaucoma are still unclear. We hypothesize that chronic oxidative stress may contribute to the malfunction of the outflow pathway by impairing the intracellular proteasome system of the cells, decreasing the ability of the tissue to modulate outflow resistance. To study the effects of chronic oxidative stress on proteasome function, primary cultures of human TM cells were incubated under 40% oxygen and proteasome activity was analyzed by measuring the accumulation of enhanced green fluorescent protein fused to a PEST motif. Changes in proteasome content, cellular senescence, and cell viability were also monitored. After 10 days of exposure to chronic oxidative stress, TM cells showed a marked decline in proteasome activity that was associated with premature senescence and decreased cell viability. These results suggest that proteasome failure may be involved in glaucoma pathophysiology.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

August 22, 2003

Volume

308

Issue

2

Start / End Page

346 / 352

Location

United States

Related Subject Headings

  • Trabecular Meshwork
  • Recombinant Fusion Proteins
  • Proteasome Endopeptidase Complex
  • Oxidative Stress
  • Multienzyme Complexes
  • Luminescent Proteins
  • Humans
  • Green Fluorescent Proteins
  • Glaucoma
  • Cysteine Endopeptidases
 

Citation

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Caballero, M., Liton, P. B., Epstein, D. L., & Gonzalez, P. (2003). Proteasome inhibition by chronic oxidative stress in human trabecular meshwork cells. Biochem Biophys Res Commun, 308(2), 346–352. https://doi.org/10.1016/s0006-291x(03)01385-8
Caballero, Montserrat, Paloma B. Liton, David L. Epstein, and Pedro Gonzalez. “Proteasome inhibition by chronic oxidative stress in human trabecular meshwork cells.Biochem Biophys Res Commun 308, no. 2 (August 22, 2003): 346–52. https://doi.org/10.1016/s0006-291x(03)01385-8.
Caballero M, Liton PB, Epstein DL, Gonzalez P. Proteasome inhibition by chronic oxidative stress in human trabecular meshwork cells. Biochem Biophys Res Commun. 2003 Aug 22;308(2):346–52.
Caballero, Montserrat, et al. “Proteasome inhibition by chronic oxidative stress in human trabecular meshwork cells.Biochem Biophys Res Commun, vol. 308, no. 2, Aug. 2003, pp. 346–52. Pubmed, doi:10.1016/s0006-291x(03)01385-8.
Caballero M, Liton PB, Epstein DL, Gonzalez P. Proteasome inhibition by chronic oxidative stress in human trabecular meshwork cells. Biochem Biophys Res Commun. 2003 Aug 22;308(2):346–352.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

August 22, 2003

Volume

308

Issue

2

Start / End Page

346 / 352

Location

United States

Related Subject Headings

  • Trabecular Meshwork
  • Recombinant Fusion Proteins
  • Proteasome Endopeptidase Complex
  • Oxidative Stress
  • Multienzyme Complexes
  • Luminescent Proteins
  • Humans
  • Green Fluorescent Proteins
  • Glaucoma
  • Cysteine Endopeptidases