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Alterations in gene expression induced by cyclic mechanical stress in trabecular meshwork cells.

Publication ,  Journal Article
Luna, C; Li, G; Liton, PB; Epstein, DL; Gonzalez, P
Published in: Mol Vis
2009

PURPOSE: To investigate the changes in gene expression induced by cyclic mechanical stress (CMS) in trabecular meshwork (TM) cells. METHODS: Human TM cultures from three donors were plated on type I collagen-coated flexible silicone bottom plates and subjected to 15% stretching, one cycle per second for 6 h. Non-stressed parallel control cultures were incubated under the same conditions in the absence of CMS. Total RNA from each culture was amplified (1 round of amplification) and hybridized to Operon Human Oligo Arrays version 3.0 (35 K probes). Differences in gene expression induced by CMS were analyzed using Genespring 7.2. quantitative polymerase chain reaction (Q-PCR) was used to confirm changes in the expressions of 12 selected genes. The effects of chemical inhibitors for p38, ERK (extracellular signal-regulated kinase), JNK (Jun N-terminal kinase), PKA (protein kinase A), PI3K (phosphoinositide 3-kinase), and P2 (purinergic 2) receptors on the induction of MMP3 (matrix metalloproteinase 3), HSP70 (heat shock protein 70), ECSM1 (endothelial cell specific molecule 1), BMP2 (bone morphogenetic protein 2), VEGFC (vascular endothelial growth factor C), and IL-8 (interleukin 8) were evaluated in porcine TM cells subjected to the same regime of CMS as that used in human cells. RESULTS: CMS induced extensive gene expression changes (664 genes, p < or = 0.05) twofold or higher in cultured TM cells. Many of these changes were related to extracellular matrix (ECM) synthesis and remodeling including the upregulation of two metalloproteinases (MMP3 and MMP10). Cytoskeleton and cell adhesion genes were also affected by CMS as well as genes known to be involved in cellular protection against stress including several members of the HSP70 family. Inhibition of PI3K/AKT and P2 receptors pathways significantly reduced the induction of MMP3 and IL-8 whereas the inhibition of the PKA/cAMP pathway decreased ECSM1 and BMP2. CONCLUSIONS: CMS activated many genes that could influence the aqueous humor outflow facility, specifically genes involved in ECM synthesis and remodeling (e.g. MMPs), cytoskeletal organization, and cell adhesion. Induction of MMP3 has the potential to increase the aqueous humor outflow facility and could be part of a homeostatic mechanism involved in the maintenance of normal intraocular pressure (IOP) levels. Other observed changes are more likely to be related to general cellular responses to stress (e.g., HSP70, ECSM1, and BMP2). Although these latter changes may initially help to repair mechanical damage, some of them such as the induction of BMP2 could eventually increase tissue rigidity and compromise the ability of the TM to maintain normal levels of outflow resistance.

Duke Scholars

Published In

Mol Vis

EISSN

1090-0535

Publication Date

2009

Volume

15

Start / End Page

534 / 544

Location

United States

Related Subject Headings

  • Trabecular Meshwork
  • Swine
  • Stress, Mechanical
  • Ophthalmology & Optometry
  • Matrix Metalloproteinase 3
  • Matrix Metalloproteinase 10
  • MAP Kinase Signaling System
  • Humans
  • HSP70 Heat-Shock Proteins
  • Gene Expression Profiling
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Luna, C., Li, G., Liton, P. B., Epstein, D. L., & Gonzalez, P. (2009). Alterations in gene expression induced by cyclic mechanical stress in trabecular meshwork cells. Mol Vis, 15, 534–544.
Luna, Coralia, Guorong Li, Paloma B. Liton, David L. Epstein, and Pedro Gonzalez. “Alterations in gene expression induced by cyclic mechanical stress in trabecular meshwork cells.Mol Vis 15 (2009): 534–44.
Luna C, Li G, Liton PB, Epstein DL, Gonzalez P. Alterations in gene expression induced by cyclic mechanical stress in trabecular meshwork cells. Mol Vis. 2009;15:534–44.
Luna, Coralia, et al. “Alterations in gene expression induced by cyclic mechanical stress in trabecular meshwork cells.Mol Vis, vol. 15, 2009, pp. 534–44.
Luna C, Li G, Liton PB, Epstein DL, Gonzalez P. Alterations in gene expression induced by cyclic mechanical stress in trabecular meshwork cells. Mol Vis. 2009;15:534–544.

Published In

Mol Vis

EISSN

1090-0535

Publication Date

2009

Volume

15

Start / End Page

534 / 544

Location

United States

Related Subject Headings

  • Trabecular Meshwork
  • Swine
  • Stress, Mechanical
  • Ophthalmology & Optometry
  • Matrix Metalloproteinase 3
  • Matrix Metalloproteinase 10
  • MAP Kinase Signaling System
  • Humans
  • HSP70 Heat-Shock Proteins
  • Gene Expression Profiling