A spectrophotometric glutathione S-transferase assay displaying alpha-class selectivity utilizing 1-p-chlorophenyl-4,4-dimethyl-5-diethylamino-1-penten-3- one hydrobromide.
Publication
, Journal Article
Sexton, DJ; Dimmock, JR; Mutus, B
Published in: Biochem Cell Biol
1993
The conjugation of 1-p-chlorophenyl-4,4-dimethyl-5-diethylamino-1-penten-3- one hydrobromide (CDDP), a Mannich base of an alpha,beta-unsaturated ketone, to glutathione is catalyzed selectively by alpha-class glutathione S-transferase. The reaction of CDDP with glutathione can be monitored continuously by following the conjugation-dependent decrease in absorbance of CDDP at 307 nm. The Km and Vmax for CDDP with alpha-class glutathione S-transferase from horse liver were determined to be 226 microM and 14.6 mumol/(min.mg), respectively. CDDP is the first example of an alpha-class glutathione S-transferase selective substrate that monitors the glutathione conjugation activity, rather than the glutathione peroxidase activity of the enzyme.
Duke Scholars
Published In
Biochem Cell Biol
DOI
ISSN
0829-8211
Publication Date
1993
Volume
71
Issue
1-2
Start / End Page
98 / 101
Location
Canada
Related Subject Headings
- Substrate Specificity
- Ketones
- Glutathione Transferase
- Glutathione
- Chlorobenzenes
- Biochemistry & Molecular Biology
- 32 Biomedical and clinical sciences
- 31 Biological sciences
- 11 Medical and Health Sciences
- 08 Information and Computing Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Sexton, D. J., Dimmock, J. R., & Mutus, B. (1993). A spectrophotometric glutathione S-transferase assay displaying alpha-class selectivity utilizing 1-p-chlorophenyl-4,4-dimethyl-5-diethylamino-1-penten-3- one hydrobromide. Biochem Cell Biol, 71(1–2), 98–101. https://doi.org/10.1139/o93-016
Sexton, D. J., J. R. Dimmock, and B. Mutus. “A spectrophotometric glutathione S-transferase assay displaying alpha-class selectivity utilizing 1-p-chlorophenyl-4,4-dimethyl-5-diethylamino-1-penten-3- one hydrobromide.” Biochem Cell Biol 71, no. 1–2 (1993): 98–101. https://doi.org/10.1139/o93-016.
Sexton DJ, Dimmock JR, Mutus B. A spectrophotometric glutathione S-transferase assay displaying alpha-class selectivity utilizing 1-p-chlorophenyl-4,4-dimethyl-5-diethylamino-1-penten-3- one hydrobromide. Biochem Cell Biol. 1993;71(1–2):98–101.
Sexton, D. J., et al. “A spectrophotometric glutathione S-transferase assay displaying alpha-class selectivity utilizing 1-p-chlorophenyl-4,4-dimethyl-5-diethylamino-1-penten-3- one hydrobromide.” Biochem Cell Biol, vol. 71, no. 1–2, 1993, pp. 98–101. Pubmed, doi:10.1139/o93-016.
Sexton DJ, Dimmock JR, Mutus B. A spectrophotometric glutathione S-transferase assay displaying alpha-class selectivity utilizing 1-p-chlorophenyl-4,4-dimethyl-5-diethylamino-1-penten-3- one hydrobromide. Biochem Cell Biol. 1993;71(1–2):98–101.
Published In
Biochem Cell Biol
DOI
ISSN
0829-8211
Publication Date
1993
Volume
71
Issue
1-2
Start / End Page
98 / 101
Location
Canada
Related Subject Headings
- Substrate Specificity
- Ketones
- Glutathione Transferase
- Glutathione
- Chlorobenzenes
- Biochemistry & Molecular Biology
- 32 Biomedical and clinical sciences
- 31 Biological sciences
- 11 Medical and Health Sciences
- 08 Information and Computing Sciences