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Evaluation of cathepsin D and epidermal growth factor receptor in prostate carcinoma.

Publication ,  Journal Article
Maygarden, SJ; Novotny, DB; Moul, JW; Bae, VL; Ware, JL
Published in: Mod Pathol
December 1994

Differential reactivity for cathepsin D (cath-D) and epidermal growth factor receptor (EGFR) was compared in 102 archival cases of human primary prostatic carcinoma and nine prostate carcinoma metastases by immunohistochemical techniques using commercially available antibodies (Ciba-Corning, Triton Diagnostics Division, Alameda, CA). Western immunoblotting confirmed that the anti-cath-D and anti-EGFR antibodies recognized the appropriate-sized proteins in extracts of human prostatic carcinoma cell lines. For immunohistochemical analysis, the primary prostate carcinomas ranged from Gleason's combined scores of 2 to 9. High-grade prostatic intraepithelial neoplasia was coexistent in 79 of the cases. Immunohistochemical staining was scored by summing the intensity of staining (0 to 3+) weighted by the percentage of tumor staining at each intensity (H score, theoretical range 0 to 300). Heterogenous moderate to strong reactivity with anti-cath-D was detected in 96 of 102 cases of primary prostate carcinoma (94%), with a mean H score of 176.5. EGFR reactivity was much less common and less strong, with 41 of 102 primary prostate carcinomas staining (40%) at a mean H score intensity of 29.2. The immunohistochemical (H) scores of cath-D and EGFR reactivity both significantly correlated with the Gleason's combined score of the tumors. There was no significant correlation between the cath-D and EGFR scores. Ninety-nine percent of the examples of prostatic intraepithelial neoplasia were reactive with anti-cath-D, with no clear correlation between the intensity of staining of prostatic intraepithelial neoplasia and the adjacent carcinoma.(ABSTRACT TRUNCATED AT 250 WORDS)

Duke Scholars

Published In

Mod Pathol

ISSN

0893-3952

Publication Date

December 1994

Volume

7

Issue

9

Start / End Page

930 / 936

Location

United States

Related Subject Headings

  • Prostatic Neoplasms
  • Pathology
  • Male
  • Immunohistochemistry
  • Humans
  • ErbB Receptors
  • Cathepsin D
  • Blotting, Western
  • Adenocarcinoma
  • 3202 Clinical sciences
 

Citation

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Maygarden, S. J., Novotny, D. B., Moul, J. W., Bae, V. L., & Ware, J. L. (1994). Evaluation of cathepsin D and epidermal growth factor receptor in prostate carcinoma. Mod Pathol, 7(9), 930–936.
Maygarden, S. J., D. B. Novotny, J. W. Moul, V. L. Bae, and J. L. Ware. “Evaluation of cathepsin D and epidermal growth factor receptor in prostate carcinoma.Mod Pathol 7, no. 9 (December 1994): 930–36.
Maygarden SJ, Novotny DB, Moul JW, Bae VL, Ware JL. Evaluation of cathepsin D and epidermal growth factor receptor in prostate carcinoma. Mod Pathol. 1994 Dec;7(9):930–6.
Maygarden, S. J., et al. “Evaluation of cathepsin D and epidermal growth factor receptor in prostate carcinoma.Mod Pathol, vol. 7, no. 9, Dec. 1994, pp. 930–36.
Maygarden SJ, Novotny DB, Moul JW, Bae VL, Ware JL. Evaluation of cathepsin D and epidermal growth factor receptor in prostate carcinoma. Mod Pathol. 1994 Dec;7(9):930–936.

Published In

Mod Pathol

ISSN

0893-3952

Publication Date

December 1994

Volume

7

Issue

9

Start / End Page

930 / 936

Location

United States

Related Subject Headings

  • Prostatic Neoplasms
  • Pathology
  • Male
  • Immunohistochemistry
  • Humans
  • ErbB Receptors
  • Cathepsin D
  • Blotting, Western
  • Adenocarcinoma
  • 3202 Clinical sciences