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PSGR, a novel prostate-specific gene with homology to a G protein-coupled receptor, is overexpressed in prostate cancer.

Publication ,  Journal Article
Xu, LL; Stackhouse, BG; Florence, K; Zhang, W; Shanmugam, N; Sesterhenn, IA; Zou, Z; Srikantan, V; Augustus, M; Roschke, V; Carter, K ...
Published in: Cancer Res
December 1, 2000

PSGR, a new prostate tissue-specific gene with homology to the G protein-coupled odorant receptor gene family, has been identified. Here we report the characteristics of the predicted protein sequence of PSGR and its prostate tissue specificity and expression profile in human prostate cancer and matched normal tissues. Using multiple tissue Northern blots from over 50 different tissues, PSGR expression was restricted to human prostate tissues. Paired normal and tumor specimens from 52 primary prostate cancers, obtained by laser capture microdissection or manual microdissection, were analyzed for PSGR expression by semiquantitative and real-time PCR assays. The differential expression of PSGR between normal and tumor tissues was highly significant (P < 0.001), and 32 of 52 (62%) matched prostate specimens exhibited tumor-associated overexpression of PSGR. Of note, there was very little or no expression of PSGR in many normal specimens in comparison with the generally high expression of PSGR seen in matched tumor specimens. In situ hybridization assays showed restricted PSGR expression in the epithelial cells of the normal and tumor tissue sections. Restricted expression of PSGR in prostatic epithelial cells, overexpression of the PSGR in a significant percentage of prostate cancers, and the predicted protein sequence of PSGR with seven transmembrane domains provide a foundation for future studies evaluating the potential of PSGR as a prostate cancer gene expression marker and the utility of PSGR protein as a novel target for developing immunotherapeutic strategies for prostate cancer.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

December 1, 2000

Volume

60

Issue

23

Start / End Page

6568 / 6572

Location

United States

Related Subject Headings

  • Sequence Homology, Amino Acid
  • Reverse Transcriptase Polymerase Chain Reaction
  • Receptors, Odorant
  • Receptors, Cell Surface
  • RNA, Messenger
  • Prostatic Neoplasms
  • Prostate
  • Phylogeny
  • Organ Specificity
  • Oncology & Carcinogenesis
 

Citation

APA
Chicago
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MLA
NLM
Xu, L. L., Stackhouse, B. G., Florence, K., Zhang, W., Shanmugam, N., Sesterhenn, I. A., … Srivastava, S. (2000). PSGR, a novel prostate-specific gene with homology to a G protein-coupled receptor, is overexpressed in prostate cancer. Cancer Res, 60(23), 6568–6572.
Xu, L. L., B. G. Stackhouse, K. Florence, W. Zhang, N. Shanmugam, I. A. Sesterhenn, Z. Zou, et al. “PSGR, a novel prostate-specific gene with homology to a G protein-coupled receptor, is overexpressed in prostate cancer.Cancer Res 60, no. 23 (December 1, 2000): 6568–72.
Xu LL, Stackhouse BG, Florence K, Zhang W, Shanmugam N, Sesterhenn IA, et al. PSGR, a novel prostate-specific gene with homology to a G protein-coupled receptor, is overexpressed in prostate cancer. Cancer Res. 2000 Dec 1;60(23):6568–72.
Xu LL, Stackhouse BG, Florence K, Zhang W, Shanmugam N, Sesterhenn IA, Zou Z, Srikantan V, Augustus M, Roschke V, Carter K, McLeod DG, Moul JW, Soppett D, Srivastava S. PSGR, a novel prostate-specific gene with homology to a G protein-coupled receptor, is overexpressed in prostate cancer. Cancer Res. 2000 Dec 1;60(23):6568–6572.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

December 1, 2000

Volume

60

Issue

23

Start / End Page

6568 / 6572

Location

United States

Related Subject Headings

  • Sequence Homology, Amino Acid
  • Reverse Transcriptase Polymerase Chain Reaction
  • Receptors, Odorant
  • Receptors, Cell Surface
  • RNA, Messenger
  • Prostatic Neoplasms
  • Prostate
  • Phylogeny
  • Organ Specificity
  • Oncology & Carcinogenesis