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Cell therapy in murine atherosclerosis: in vivo imaging with high-resolution helical SPECT.

Publication ,  Journal Article
Vemulapalli, S; Metzler, SD; Akabani, G; Petry, NA; Niehaus, NJ; Liu, X; Patil, NH; Greer, KL; Jaszczak, RJ; Coleman, RE; Dong, C; Chin, BB ...
Published in: Radiology
January 2007

PURPOSE: To determine the feasibility of in vivo localization and quantification of indium 111 (111In)-oxine-labeled bone marrow (BM) with high-resolution whole-body helical single photon emission computed tomography (SPECT) in an established murine model of atherosclerosis and vascular repair. MATERIALS AND METHODS: The institutional animal care and use committee approved this study. BM from young B6 Rosa 26 Lac Z+/+ mice was radiolabeled with 111In-oxine. On days 1, 4, and 7 after administration of radiolabeled cells, five C57/BL6 apolipoprotein E-deficient mice and five wild-type (WT) control mice were imaged with whole-body high-resolution helical SPECT. Quantification with SPECT was compared with ex vivo analysis by means of gamma counting. Autoradiography and beta-galactosidase staining were used to verify donor cell biodistribution. Linear regression was used to assess the correlation between continuous variables. Two-tailed Student t test was used to compare values between groups, and paired two-tailed t test was used to assess changes within subjects at different time points. RESULTS: SPECT image contrast was high, with clear visualization of BM, liver, and spleen 7 days after administration of radiolabeled cells. SPECT revealed that 42% and 58% more activity was localized to the aorta and BM (P<.05 for both), respectively, in apolipoprotein E-deficient mice versus WT mice. Furthermore, 28% and 27% less activity was localized to the liver and spleen (P<.05 for both), respectively, in apolipoprotein E-deficient mice versus WT mice. SPECT and organ gamma counts showed good quantitative correlation (r=0.9). beta-Galactosidase staining and microautoradiography of recipient aortas showed donor cell localization to the intima of visible atherosclerotic plaque but not to unaffected regions of the vessel wall. CONCLUSION: High-resolution in vivo helical pinhole SPECT can be used to monitor and quantify early biodistribution of 111In-oxine-labeled BM in a murine model of progenitor cell therapy for atherosclerosis.

Duke Scholars

Published In

Radiology

DOI

ISSN

0033-8419

Publication Date

January 2007

Volume

242

Issue

1

Start / End Page

198 / 207

Location

United States

Related Subject Headings

  • Tomography, Emission-Computed, Single-Photon
  • Radiopharmaceuticals
  • Oxyquinoline
  • Organometallic Compounds
  • Nuclear Medicine & Medical Imaging
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Image Enhancement
  • Feasibility Studies
 

Citation

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ICMJE
MLA
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Vemulapalli, S., Metzler, S. D., Akabani, G., Petry, N. A., Niehaus, N. J., Liu, X., … Chin, B. B. (2007). Cell therapy in murine atherosclerosis: in vivo imaging with high-resolution helical SPECT. Radiology, 242(1), 198–207. https://doi.org/10.1148/radiol.2421051461
Vemulapalli, Sreekanth, Scott D. Metzler, Gamal Akabani, Neil A. Petry, Nelsen J. Niehaus, Xialin Liu, Nikhil H. Patil, et al. “Cell therapy in murine atherosclerosis: in vivo imaging with high-resolution helical SPECT.Radiology 242, no. 1 (January 2007): 198–207. https://doi.org/10.1148/radiol.2421051461.
Vemulapalli S, Metzler SD, Akabani G, Petry NA, Niehaus NJ, Liu X, et al. Cell therapy in murine atherosclerosis: in vivo imaging with high-resolution helical SPECT. Radiology. 2007 Jan;242(1):198–207.
Vemulapalli, Sreekanth, et al. “Cell therapy in murine atherosclerosis: in vivo imaging with high-resolution helical SPECT.Radiology, vol. 242, no. 1, Jan. 2007, pp. 198–207. Pubmed, doi:10.1148/radiol.2421051461.
Vemulapalli S, Metzler SD, Akabani G, Petry NA, Niehaus NJ, Liu X, Patil NH, Greer KL, Jaszczak RJ, Coleman RE, Dong C, Goldschmidt-Clermont PJ, Chin BB. Cell therapy in murine atherosclerosis: in vivo imaging with high-resolution helical SPECT. Radiology. 2007 Jan;242(1):198–207.
Journal cover image

Published In

Radiology

DOI

ISSN

0033-8419

Publication Date

January 2007

Volume

242

Issue

1

Start / End Page

198 / 207

Location

United States

Related Subject Headings

  • Tomography, Emission-Computed, Single-Photon
  • Radiopharmaceuticals
  • Oxyquinoline
  • Organometallic Compounds
  • Nuclear Medicine & Medical Imaging
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Image Enhancement
  • Feasibility Studies