Interferon regulatory factors are transcriptional regulators of adipogenesis.
We have sought to identify transcriptional pathways in adipogenesis using an integrated experimental and computational approach. Here, we employ high-throughput DNase hypersensitivity analysis to find regions of altered chromatin structure surrounding key adipocyte genes. Regions that display differentiation-dependent changes in hypersensitivity were used to predict binding sites for proteins involved in adipogenesis. A high-scoring example was a binding motif for interferon regulatory factor (IRF) family members. Expression of all nine mammalian IRF mRNAs is regulated during adipogenesis, and several bind to the identified motifs in a differentiation-dependent manner. Furthermore, several IRF proteins repress differentiation. This analysis suggests an important role for IRF proteins in adipocyte biology and demonstrates the utility of this approach in identifying cis- and trans-acting factors not previously suspected to participate in adipogenesis.
Duke Scholars
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Related Subject Headings
- Transcription, Genetic
- Protein Binding
- Polymerase Chain Reaction
- Mice
- Interferon Regulatory Factors
- Gene Expression Regulation
- Endocrinology & Metabolism
- Electrophoretic Mobility Shift Assay
- Deoxyribonucleases
- Chromatin Immunoprecipitation
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Transcription, Genetic
- Protein Binding
- Polymerase Chain Reaction
- Mice
- Interferon Regulatory Factors
- Gene Expression Regulation
- Endocrinology & Metabolism
- Electrophoretic Mobility Shift Assay
- Deoxyribonucleases
- Chromatin Immunoprecipitation