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An improved method for glycosaminoglycan analysis by LC-MS/MS of urine samples collected on filter paper.

Publication ,  Journal Article
Auray-Blais, C; Lavoie, P; Zhang, H; Gagnon, R; Clarke, JTR; Maranda, B; Young, SP; An, Y; Millington, DS
Published in: Clin Chim Acta
April 11, 2012

BACKGROUND: Mucopolysaccharidoses are complex lysosomal storage disorders caused by any of eleven different enzyme deficiencies resulting in the accumulation of substrates, mainly glycosaminoglycans (GAGs), in various tissues and biological fluids. METHOD: We developed and validated a urine filter paper methodology for the analysis of GAGs using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for mucopolysaccharidoses type I, type II and type VI patients. We focused on 2 objectives: first, its applicability to high-risk screening, and secondly, to facilitate the collection and shipping of samples to reference centers as part of diagnostic investigation, as well as from treated patients needing to be monitored for assessment of the efficacy of treatment. GAGs in urine dried onto filter paper were extracted and subjected to methanolysis to obtain the repeating disaccharides of the molecules. We devised a multiple reaction monitoring method in positive electrospray ionization mode. RESULTS: The use of deuterated internal standards for dermatan sulfate (DS) and heparan sulfate (HS) reduced a troubling matrix effect. The resulting CVs were <14%. Linearity assessment showed Pearson correlation coefficients of 0.999 and 0.997, for DS and HS, respectively. The stability on filter paper was good for DS and HS for up to 6 weeks at various temperatures. CONCLUSION: We devised a robust and efficient LC-MS/MS methodology for GAGS quantification in urine dried on filter paper and subjected to environmental conditions likely to be encountered during collection, storage and shipping of specimens from referring physicians to medical centers.

Duke Scholars

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Published In

Clin Chim Acta

DOI

EISSN

1873-3492

Publication Date

April 11, 2012

Volume

413

Issue

7-8

Start / End Page

771 / 778

Location

Netherlands

Related Subject Headings

  • Tandem Mass Spectrometry
  • Reference Standards
  • Paper
  • Humans
  • Glycosaminoglycans
  • General Clinical Medicine
  • Chromatography, Liquid
  • 3202 Clinical sciences
  • 3101 Biochemistry and cell biology
  • 1103 Clinical Sciences
 

Citation

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Auray-Blais, C., Lavoie, P., Zhang, H., Gagnon, R., Clarke, J. T. R., Maranda, B., … Millington, D. S. (2012). An improved method for glycosaminoglycan analysis by LC-MS/MS of urine samples collected on filter paper. Clin Chim Acta, 413(7–8), 771–778. https://doi.org/10.1016/j.cca.2012.01.012
Auray-Blais, Christiane, Pamela Lavoie, Haoyue Zhang, René Gagnon, Joe T. R. Clarke, Bruno Maranda, Sarah P. Young, Yan An, and David S. Millington. “An improved method for glycosaminoglycan analysis by LC-MS/MS of urine samples collected on filter paper.Clin Chim Acta 413, no. 7–8 (April 11, 2012): 771–78. https://doi.org/10.1016/j.cca.2012.01.012.
Auray-Blais C, Lavoie P, Zhang H, Gagnon R, Clarke JTR, Maranda B, et al. An improved method for glycosaminoglycan analysis by LC-MS/MS of urine samples collected on filter paper. Clin Chim Acta. 2012 Apr 11;413(7–8):771–8.
Auray-Blais, Christiane, et al. “An improved method for glycosaminoglycan analysis by LC-MS/MS of urine samples collected on filter paper.Clin Chim Acta, vol. 413, no. 7–8, Apr. 2012, pp. 771–78. Pubmed, doi:10.1016/j.cca.2012.01.012.
Auray-Blais C, Lavoie P, Zhang H, Gagnon R, Clarke JTR, Maranda B, Young SP, An Y, Millington DS. An improved method for glycosaminoglycan analysis by LC-MS/MS of urine samples collected on filter paper. Clin Chim Acta. 2012 Apr 11;413(7–8):771–778.
Journal cover image

Published In

Clin Chim Acta

DOI

EISSN

1873-3492

Publication Date

April 11, 2012

Volume

413

Issue

7-8

Start / End Page

771 / 778

Location

Netherlands

Related Subject Headings

  • Tandem Mass Spectrometry
  • Reference Standards
  • Paper
  • Humans
  • Glycosaminoglycans
  • General Clinical Medicine
  • Chromatography, Liquid
  • 3202 Clinical sciences
  • 3101 Biochemistry and cell biology
  • 1103 Clinical Sciences