Skip to main content
Journal cover image

Analysis of bacterial cultures in infectious keratitis, 1997 to 2004.

Publication ,  Journal Article
Yeh, DL; Stinnett, SS; Afshari, NA
Published in: Am J Ophthalmol
December 2006

PURPOSE: To review bacterial culture results in infectious keratitis at a tertiary referral center. DESIGN: Retrospective case series. METHODS: Analysis of Gram stain, bacterial culture, and targeted antibiotic sensitivities of all cases of presumed infectious keratitis at Duke University Eye Center from 1997 to 2004 (n = 453). RESULTS: Cultures were positive in 307 cases (68%); 21% demonstrated polymicrobial growth. Among 388 isolates, 81% were Gram-positive. Gram stain results were available in 334 cases, demonstrating 18% sensitivity and 94% specificity. Antibiotic sensitivities revealed 24% resistance of tested S. aureus isolates to cefazolin, and <5% resistance of tested gram-negative isolates to various antibiotics, with no statistically significant increase from 1997 to 2004. CONCLUSIONS: In this study, the microbial profile has remained stable from 1997 to 2004. Gram stain studies were of poor sensitivity, while culture yields were significantly higher and demonstrated a relatively high polymicrobial infection rate. Increasing antibiotic resistance was not clearly demonstrated.

Duke Scholars

Published In

Am J Ophthalmol

DOI

ISSN

0002-9394

Publication Date

December 2006

Volume

142

Issue

6

Start / End Page

1066 / 1068

Location

United States

Related Subject Headings

  • Retrospective Studies
  • Ophthalmology & Optometry
  • Microbial Sensitivity Tests
  • Keratitis
  • Humans
  • Gram-Positive Bacteria
  • Gram-Negative Bacteria
  • Eye Infections, Bacterial
  • Drug Resistance, Microbial
  • Anti-Bacterial Agents
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Yeh, D. L., Stinnett, S. S., & Afshari, N. A. (2006). Analysis of bacterial cultures in infectious keratitis, 1997 to 2004. Am J Ophthalmol, 142(6), 1066–1068. https://doi.org/10.1016/j.ajo.2006.06.056
Yeh, David L., Sandra S. Stinnett, and Natalie A. Afshari. “Analysis of bacterial cultures in infectious keratitis, 1997 to 2004.Am J Ophthalmol 142, no. 6 (December 2006): 1066–68. https://doi.org/10.1016/j.ajo.2006.06.056.
Yeh DL, Stinnett SS, Afshari NA. Analysis of bacterial cultures in infectious keratitis, 1997 to 2004. Am J Ophthalmol. 2006 Dec;142(6):1066–8.
Yeh, David L., et al. “Analysis of bacterial cultures in infectious keratitis, 1997 to 2004.Am J Ophthalmol, vol. 142, no. 6, Dec. 2006, pp. 1066–68. Pubmed, doi:10.1016/j.ajo.2006.06.056.
Yeh DL, Stinnett SS, Afshari NA. Analysis of bacterial cultures in infectious keratitis, 1997 to 2004. Am J Ophthalmol. 2006 Dec;142(6):1066–1068.
Journal cover image

Published In

Am J Ophthalmol

DOI

ISSN

0002-9394

Publication Date

December 2006

Volume

142

Issue

6

Start / End Page

1066 / 1068

Location

United States

Related Subject Headings

  • Retrospective Studies
  • Ophthalmology & Optometry
  • Microbial Sensitivity Tests
  • Keratitis
  • Humans
  • Gram-Positive Bacteria
  • Gram-Negative Bacteria
  • Eye Infections, Bacterial
  • Drug Resistance, Microbial
  • Anti-Bacterial Agents