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Aedes albopictus cells resistant to adenosine because of a defect in nucleoside transport.

Publication ,  Journal Article
Sherwood, A; Stollar, V
Published in: Somatic Cell Genet
September 1982

By growing Aedes albopictus mosquito cells in media containing increasing concentrations of adenosine and subsequently plating low numbers of cells in the presence of EHNA (an inhibitor of adenosine deaminase), three clones were obtained which were resistant to adenosine. The adenosine-resistant clones contained level of adenosine and thymidine kinase similar to those in the parental cells, but were unable to incorporate labeled nucleotides (adenosine, uridine, thymidine, or guanosine) into TCA-precipitable material. The inability to incorporate nucleosides was also reflected in an enhanced resistance to several nucleoside analogs such as 5-fluorodeoxyuridine and tubercidin but not to the unribosylated base, 5-fluorouracil. Direct measurements over short time intervals indicated that the primary defect in these cells was at the level of nucleoside transport.

Duke Scholars

Published In

Somatic Cell Genet

DOI

ISSN

0098-0366

Publication Date

September 1982

Volume

8

Issue

5

Start / End Page

575 / 585

Location

United States

Related Subject Headings

  • Thymidine Kinase
  • Nucleosides
  • Drug Resistance
  • Clone Cells
  • Biological Transport, Active
  • Animals
  • Aedes
  • Adenosine Kinase
  • Adenosine
 

Citation

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MLA
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Sherwood, A., & Stollar, V. (1982). Aedes albopictus cells resistant to adenosine because of a defect in nucleoside transport. Somatic Cell Genet, 8(5), 575–585. https://doi.org/10.1007/BF01542852
Sherwood, A., and V. Stollar. “Aedes albopictus cells resistant to adenosine because of a defect in nucleoside transport.Somatic Cell Genet 8, no. 5 (September 1982): 575–85. https://doi.org/10.1007/BF01542852.
Sherwood A, Stollar V. Aedes albopictus cells resistant to adenosine because of a defect in nucleoside transport. Somatic Cell Genet. 1982 Sep;8(5):575–85.
Sherwood, A., and V. Stollar. “Aedes albopictus cells resistant to adenosine because of a defect in nucleoside transport.Somatic Cell Genet, vol. 8, no. 5, Sept. 1982, pp. 575–85. Pubmed, doi:10.1007/BF01542852.
Sherwood A, Stollar V. Aedes albopictus cells resistant to adenosine because of a defect in nucleoside transport. Somatic Cell Genet. 1982 Sep;8(5):575–585.

Published In

Somatic Cell Genet

DOI

ISSN

0098-0366

Publication Date

September 1982

Volume

8

Issue

5

Start / End Page

575 / 585

Location

United States

Related Subject Headings

  • Thymidine Kinase
  • Nucleosides
  • Drug Resistance
  • Clone Cells
  • Biological Transport, Active
  • Animals
  • Aedes
  • Adenosine Kinase
  • Adenosine