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Functional impairment in protein kinase C by RACK1 (receptor for activated C kinase 1) deficiency in aged rat brain cortex.

Publication ,  Journal Article
Pascale, A; Fortino, I; Govoni, S; Trabucchi, M; Wetsel, WC; Battaini, F
Published in: J Neurochem
December 1996

Several laboratories have reported a lack of protein kinase C (PKC) activation in response to various stimuli in the brain of aged rats. It has been suggested that changes in lipid membrane composition could be related to this functional deficit. However, recent evidence has indicated that the translocation of PKC to the different subcellular compartments is controlled by protein-protein interactions. Recently, a class of proteins, termed receptors for activated C kinase (RACKs), have been described that bind PKC. The present study was conducted to determine whether alterations in RACK1, the best-characterized member of RACKs, were associated with changes in translocation and expression of PKC. Quantitative immunoblotting revealed that RACK1 content was decreased by approximately 50% in aged rat brain cortex, compared with that in adult and middle-aged animals. The levels of calcium-independent PKC delta and epsilon, interacting with RACK1, and related calcium-independent PKC activity were not modified by the aging process. By comparison, phorbol ester-stimulated translocation of this activity and of PKC delta and epsilon immunoreactivity was absent in cortex from aged animals, as well as the translocation of the calcium-dependent PKC beta, also known to interact with RACK1. These results indicate that a deficit in RACK1 may contribute to the functional impairment in PKC activation observed in aged rat brain.

Duke Scholars

Published In

J Neurochem

DOI

ISSN

0022-3042

Publication Date

December 1996

Volume

67

Issue

6

Start / End Page

2471 / 2477

Location

England

Related Subject Headings

  • Tetradecanoylphorbol Acetate
  • Receptors, Cell Surface
  • Receptors for Activated C Kinase
  • Rats, Wistar
  • Rats
  • Protein Kinase C-epsilon
  • Protein Kinase C-delta
  • Protein Kinase C beta
  • Protein Kinase C
  • Peptides
 

Citation

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MLA
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Pascale, A., Fortino, I., Govoni, S., Trabucchi, M., Wetsel, W. C., & Battaini, F. (1996). Functional impairment in protein kinase C by RACK1 (receptor for activated C kinase 1) deficiency in aged rat brain cortex. J Neurochem, 67(6), 2471–2477. https://doi.org/10.1046/j.1471-4159.1996.67062471.x
Pascale, A., I. Fortino, S. Govoni, M. Trabucchi, W. C. Wetsel, and F. Battaini. “Functional impairment in protein kinase C by RACK1 (receptor for activated C kinase 1) deficiency in aged rat brain cortex.J Neurochem 67, no. 6 (December 1996): 2471–77. https://doi.org/10.1046/j.1471-4159.1996.67062471.x.
Pascale A, Fortino I, Govoni S, Trabucchi M, Wetsel WC, Battaini F. Functional impairment in protein kinase C by RACK1 (receptor for activated C kinase 1) deficiency in aged rat brain cortex. J Neurochem. 1996 Dec;67(6):2471–7.
Pascale, A., et al. “Functional impairment in protein kinase C by RACK1 (receptor for activated C kinase 1) deficiency in aged rat brain cortex.J Neurochem, vol. 67, no. 6, Dec. 1996, pp. 2471–77. Pubmed, doi:10.1046/j.1471-4159.1996.67062471.x.
Pascale A, Fortino I, Govoni S, Trabucchi M, Wetsel WC, Battaini F. Functional impairment in protein kinase C by RACK1 (receptor for activated C kinase 1) deficiency in aged rat brain cortex. J Neurochem. 1996 Dec;67(6):2471–2477.
Journal cover image

Published In

J Neurochem

DOI

ISSN

0022-3042

Publication Date

December 1996

Volume

67

Issue

6

Start / End Page

2471 / 2477

Location

England

Related Subject Headings

  • Tetradecanoylphorbol Acetate
  • Receptors, Cell Surface
  • Receptors for Activated C Kinase
  • Rats, Wistar
  • Rats
  • Protein Kinase C-epsilon
  • Protein Kinase C-delta
  • Protein Kinase C beta
  • Protein Kinase C
  • Peptides