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Expression of candidate pro-GnRH processing enzymes in rat hypothalamus and an immortalized hypothalamic neuronal cell line.

Publication ,  Journal Article
Wetsel, WC; Liposits, Z; Seidah, NG; Collins, S
Published in: Neuroendocrinology
August 1995

Since gonadotropin-releasing hormone (GnRH, also referred to as LHRH) is a major hormone regulating mammalian reproduction, identification of the processing steps involved in the conversion of the pro-LHRH to LHRH is fundamental to our understanding of its physiology. Extracts from immortalized LHRH neurons (GT1) were used to isolate the pro-LHRH intermediate products and to identify the enzymes which may participate in these conversions. The GT1 cells contain and secrete a pro-LHRH species that elutes at approximately 10,000-12,000 molecular weight. The pro-LHRH is metabolized to various N- and C-terminally modified LHRH products and to gonadotropin-releasing hormone-associated peptide (GAP). Analyses of these intermediates suggests that, at least, four different enzymatic steps are involved in pro-LHRH processing. Northern blot analyses reveal that prohormone convertase 2 (PC2), carboxypeptidase E, glutaminyl cyclase, and peptidyl-glycine alpha-amidating monooxygenase are expressed in the GT1 cells and rat hypothalamus. PC2 immunoreactivity is localized to the perikarya and beaded axon-like processes of these cells. SDS-PAGE analyses indicate that PC2 is biosynthesized, processed and secreted by the immortalized LHRH neurons. Our results indicate that the GT1 cell line may serve as a useful model to study the regulation of pro-LHRH processing and that it may also represent an important tool for dissecting the molecular and cellular basis of mammalian reproduction.

Duke Scholars

Published In

Neuroendocrinology

DOI

ISSN

0028-3835

Publication Date

August 1995

Volume

62

Issue

2

Start / End Page

166 / 177

Location

Switzerland

Related Subject Headings

  • Subtilisins
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Protein Precursors
  • Proprotein Convertase 2
  • Neurons
  • Multienzyme Complexes
  • Molecular Weight
  • Molecular Sequence Data
 

Citation

APA
Chicago
ICMJE
MLA
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Wetsel, W. C., Liposits, Z., Seidah, N. G., & Collins, S. (1995). Expression of candidate pro-GnRH processing enzymes in rat hypothalamus and an immortalized hypothalamic neuronal cell line. Neuroendocrinology, 62(2), 166–177. https://doi.org/10.1159/000127001
Wetsel, W. C., Z. Liposits, N. G. Seidah, and S. Collins. “Expression of candidate pro-GnRH processing enzymes in rat hypothalamus and an immortalized hypothalamic neuronal cell line.Neuroendocrinology 62, no. 2 (August 1995): 166–77. https://doi.org/10.1159/000127001.
Wetsel WC, Liposits Z, Seidah NG, Collins S. Expression of candidate pro-GnRH processing enzymes in rat hypothalamus and an immortalized hypothalamic neuronal cell line. Neuroendocrinology. 1995 Aug;62(2):166–77.
Wetsel, W. C., et al. “Expression of candidate pro-GnRH processing enzymes in rat hypothalamus and an immortalized hypothalamic neuronal cell line.Neuroendocrinology, vol. 62, no. 2, Aug. 1995, pp. 166–77. Pubmed, doi:10.1159/000127001.
Wetsel WC, Liposits Z, Seidah NG, Collins S. Expression of candidate pro-GnRH processing enzymes in rat hypothalamus and an immortalized hypothalamic neuronal cell line. Neuroendocrinology. 1995 Aug;62(2):166–177.
Journal cover image

Published In

Neuroendocrinology

DOI

ISSN

0028-3835

Publication Date

August 1995

Volume

62

Issue

2

Start / End Page

166 / 177

Location

Switzerland

Related Subject Headings

  • Subtilisins
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Protein Precursors
  • Proprotein Convertase 2
  • Neurons
  • Multienzyme Complexes
  • Molecular Weight
  • Molecular Sequence Data