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Rapid 16S ribosomal DNA sequencing from a single colony without DNA extraction or purification.

Publication ,  Journal Article
Frothingham, R; Allen, RL; Wilson, KH
Published in: Biotechniques
July 1991

Ribosomal RNA sequences are useful for establishing phylogenetic relationships, for oligonucleotide probes and for characterization of uncultured organisms. We describe rapid ribosomal DNA sequencing using PCR with transcript sequencing. Nucleic acid specificity at three steps (amplification, transcription and sequencing) eliminated the need for nucleic acid extraction or purification. Sequence was obtained from a crude lysate from a single colony of bacteria. The basic sequencing method should be adaptable to provide rapid sequence information in a wide variety of applications.

Duke Scholars

Published In

Biotechniques

ISSN

0736-6205

Publication Date

July 1991

Volume

11

Issue

1

Start / End Page

40 / 44

Location

England

Related Subject Headings

  • Transcription, Genetic
  • RNA-Directed DNA Polymerase
  • RNA, Ribosomal, 16S
  • Polymerase Chain Reaction
  • Molecular Sequence Data
  • Escherichia coli
  • Deoxyribonucleotides
  • DNA, Ribosomal
  • DNA, Bacterial
  • Bioinformatics
 

Citation

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MLA
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Frothingham, R., Allen, R. L., & Wilson, K. H. (1991). Rapid 16S ribosomal DNA sequencing from a single colony without DNA extraction or purification. Biotechniques, 11(1), 40–44.
Frothingham, R., R. L. Allen, and K. H. Wilson. “Rapid 16S ribosomal DNA sequencing from a single colony without DNA extraction or purification.Biotechniques 11, no. 1 (July 1991): 40–44.
Frothingham R, Allen RL, Wilson KH. Rapid 16S ribosomal DNA sequencing from a single colony without DNA extraction or purification. Biotechniques. 1991 Jul;11(1):40–4.
Frothingham, R., et al. “Rapid 16S ribosomal DNA sequencing from a single colony without DNA extraction or purification.Biotechniques, vol. 11, no. 1, July 1991, pp. 40–44.
Frothingham R, Allen RL, Wilson KH. Rapid 16S ribosomal DNA sequencing from a single colony without DNA extraction or purification. Biotechniques. 1991 Jul;11(1):40–44.

Published In

Biotechniques

ISSN

0736-6205

Publication Date

July 1991

Volume

11

Issue

1

Start / End Page

40 / 44

Location

England

Related Subject Headings

  • Transcription, Genetic
  • RNA-Directed DNA Polymerase
  • RNA, Ribosomal, 16S
  • Polymerase Chain Reaction
  • Molecular Sequence Data
  • Escherichia coli
  • Deoxyribonucleotides
  • DNA, Ribosomal
  • DNA, Bacterial
  • Bioinformatics