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A quantitative method for measuring gene expression magnitude and volume delivered by gene therapy vectors.

Publication ,  Journal Article
Barton, KN; Xia, X; Yan, H; Stricker, H; Heisey, G; Yin, F-F; Nagaraja, TN; Zhu, G; Kolozsvary, A; Fenstermacher, JD; Lu, M; Kim, JH ...
Published in: Mol Ther
April 2004

This study describes a quantitative method to measure the magnitude and distribution of gene expression following local delivery of an adenoviral vector containing the human sodium iodide symporter (hNIS) reporter gene into the canine prostate. Following systemic administration of Na(99m)TcO(4), autoradiographs of prostate sections depicting hNIS-dependent (99m)TcO(4)(-) uptake were digitized and stacked to produce a three-dimensional reconstruction of gene expression. Frequency histograms reflecting hNIS gene expression magnitude and volume were used to quantify hNIS function. The method demonstrated submillimeter resolution allowing for precise measurements of gene expression magnitude and volume in vivo. The method developed here could be applied to other reporter gene systems in which the readout can be digitized from thin tissue sections.

Duke Scholars

Published In

Mol Ther

DOI

ISSN

1525-0016

Publication Date

April 2004

Volume

9

Issue

4

Start / End Page

625 / 631

Location

United States

Related Subject Headings

  • Technetium
  • Sodium Iodide
  • Prostate
  • Male
  • In Situ Hybridization
  • Humans
  • Genetic Vectors
  • Genetic Therapy
  • Genetic Techniques
  • Genes, Reporter
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Barton, K. N., Xia, X., Yan, H., Stricker, H., Heisey, G., Yin, F.-F., … Brown, S. L. (2004). A quantitative method for measuring gene expression magnitude and volume delivered by gene therapy vectors. Mol Ther, 9(4), 625–631. https://doi.org/10.1016/j.ymthe.2004.01.011
Barton, Kenneth N., Xueqing Xia, Hui Yan, Hans Stricker, Gregory Heisey, Fang-Fang Yin, Tavarekere N. Nagaraja, et al. “A quantitative method for measuring gene expression magnitude and volume delivered by gene therapy vectors.Mol Ther 9, no. 4 (April 2004): 625–31. https://doi.org/10.1016/j.ymthe.2004.01.011.
Barton KN, Xia X, Yan H, Stricker H, Heisey G, Yin F-F, et al. A quantitative method for measuring gene expression magnitude and volume delivered by gene therapy vectors. Mol Ther. 2004 Apr;9(4):625–31.
Barton, Kenneth N., et al. “A quantitative method for measuring gene expression magnitude and volume delivered by gene therapy vectors.Mol Ther, vol. 9, no. 4, Apr. 2004, pp. 625–31. Pubmed, doi:10.1016/j.ymthe.2004.01.011.
Barton KN, Xia X, Yan H, Stricker H, Heisey G, Yin F-F, Nagaraja TN, Zhu G, Kolozsvary A, Fenstermacher JD, Lu M, Kim JH, Freytag SO, Brown SL. A quantitative method for measuring gene expression magnitude and volume delivered by gene therapy vectors. Mol Ther. 2004 Apr;9(4):625–631.
Journal cover image

Published In

Mol Ther

DOI

ISSN

1525-0016

Publication Date

April 2004

Volume

9

Issue

4

Start / End Page

625 / 631

Location

United States

Related Subject Headings

  • Technetium
  • Sodium Iodide
  • Prostate
  • Male
  • In Situ Hybridization
  • Humans
  • Genetic Vectors
  • Genetic Therapy
  • Genetic Techniques
  • Genes, Reporter