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Hypocotyl transcriptome reveals auxin regulation of growth-promoting genes through GA-dependent and -independent pathways.

Publication ,  Journal Article
Chapman, EJ; Greenham, K; Castillejo, C; Sartor, R; Bialy, A; Sun, T-P; Estelle, M
Published in: PloS one
January 2012

Many processes critical to plant growth and development are regulated by the hormone auxin. Auxin responses are initiated through activation of a transcriptional response mediated by the TIR1/AFB family of F-box protein auxin receptors as well as the AUX/IAA and ARF families of transcriptional regulators. However, there is little information on how auxin regulates a specific cellular response. To begin to address this question, we have focused on auxin regulation of cell expansion in the Arabidopsis hypocotyl. We show that auxin-mediated hypocotyl elongation is dependent upon the TIR1/AFB family of auxin receptors and degradation of AUX/IAA repressors. We also use microarray studies of elongating hypocotyls to show that a number of growth-associated processes are activated by auxin including gibberellin biosynthesis, cell wall reorganization and biogenesis, and others. Our studies indicate that GA biosynthesis is required for normal response to auxin in the hypocotyl but that the overall transcriptional auxin output consists of PIF-dependent and -independent genes. We propose that auxin acts independently from and interdependently with PIF and GA pathways to regulate expression of growth-associated genes in cell expansion.

Duke Scholars

Published In

PloS one

DOI

EISSN

1932-6203

ISSN

1932-6203

Publication Date

January 2012

Volume

7

Issue

5

Start / End Page

e36210

Related Subject Headings

  • Receptors, Cell Surface
  • Plant Proteins
  • Oligonucleotide Array Sequence Analysis
  • Indoleacetic Acids
  • Hypocotyl
  • General Science & Technology
  • Gene Expression Regulation, Plant
  • Gene Expression Profiling
  • F-Box Proteins
  • Arabidopsis Proteins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Chapman, E. J., Greenham, K., Castillejo, C., Sartor, R., Bialy, A., Sun, T.-P., & Estelle, M. (2012). Hypocotyl transcriptome reveals auxin regulation of growth-promoting genes through GA-dependent and -independent pathways. PloS One, 7(5), e36210. https://doi.org/10.1371/journal.pone.0036210
Chapman, Elisabeth J., Kathleen Greenham, Cristina Castillejo, Ryan Sartor, Agniezska Bialy, Tai-Ping Sun, and Mark Estelle. “Hypocotyl transcriptome reveals auxin regulation of growth-promoting genes through GA-dependent and -independent pathways.PloS One 7, no. 5 (January 2012): e36210. https://doi.org/10.1371/journal.pone.0036210.
Chapman EJ, Greenham K, Castillejo C, Sartor R, Bialy A, Sun T-P, et al. Hypocotyl transcriptome reveals auxin regulation of growth-promoting genes through GA-dependent and -independent pathways. PloS one. 2012 Jan;7(5):e36210.
Chapman, Elisabeth J., et al. “Hypocotyl transcriptome reveals auxin regulation of growth-promoting genes through GA-dependent and -independent pathways.PloS One, vol. 7, no. 5, Jan. 2012, p. e36210. Epmc, doi:10.1371/journal.pone.0036210.
Chapman EJ, Greenham K, Castillejo C, Sartor R, Bialy A, Sun T-P, Estelle M. Hypocotyl transcriptome reveals auxin regulation of growth-promoting genes through GA-dependent and -independent pathways. PloS one. 2012 Jan;7(5):e36210.

Published In

PloS one

DOI

EISSN

1932-6203

ISSN

1932-6203

Publication Date

January 2012

Volume

7

Issue

5

Start / End Page

e36210

Related Subject Headings

  • Receptors, Cell Surface
  • Plant Proteins
  • Oligonucleotide Array Sequence Analysis
  • Indoleacetic Acids
  • Hypocotyl
  • General Science & Technology
  • Gene Expression Regulation, Plant
  • Gene Expression Profiling
  • F-Box Proteins
  • Arabidopsis Proteins