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Normal spastin gene dosage is specifically required for axon regeneration.

Publication ,  Journal Article
Stone, MC; Rao, K; Gheres, KW; Kim, S; Tao, J; La Rochelle, C; Folker, CT; Sherwood, NT; Rolls, MM
Published in: Cell reports
November 2012

Axon regeneration allows neurons to repair circuits after trauma; however, most of the molecular players in this process remain to be identified. Given that microtubule rearrangements have been observed in injured neurons, we tested whether microtubule-severing proteins might play a role in axon regeneration. We found that axon regeneration is extremely sensitive to levels of the microtubule-severing protein spastin. Although microtubule behavior in uninjured neurons was not perturbed in animals heterozygous for a spastin null allele, axon regeneration was severely disrupted in this background. Two types of axon regeneration-regeneration of an axon from a dendrite after proximal axotomy and regeneration of an axon from the stump after distal axotomy-were defective in Drosophila with one mutant copy of the spastin gene. Other types of axon and dendrite outgrowth, including regrowth of dendrites after pruning, were normal in heterozygotes. We conclude that regenerative axon growth is uniquely sensitive to spastin gene dosage.

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Published In

Cell reports

DOI

EISSN

2211-1247

ISSN

2211-1247

Publication Date

November 2012

Volume

2

Issue

5

Start / End Page

1340 / 1350

Related Subject Headings

  • Tubulin
  • RNA, Small Interfering
  • RNA Interference
  • Nerve Regeneration
  • Mutation
  • Microtubules
  • Katanin
  • Gene Dosage
  • Drosophila Proteins
  • Drosophila
 

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Stone, M. C., Rao, K., Gheres, K. W., Kim, S., Tao, J., La Rochelle, C., … Rolls, M. M. (2012). Normal spastin gene dosage is specifically required for axon regeneration. Cell Reports, 2(5), 1340–1350. https://doi.org/10.1016/j.celrep.2012.09.032
Stone, Michelle C., Kavitha Rao, Kyle W. Gheres, Seahee Kim, Juan Tao, Caroline La Rochelle, Christin T. Folker, Nina T. Sherwood, and Melissa M. Rolls. “Normal spastin gene dosage is specifically required for axon regeneration.Cell Reports 2, no. 5 (November 2012): 1340–50. https://doi.org/10.1016/j.celrep.2012.09.032.
Stone MC, Rao K, Gheres KW, Kim S, Tao J, La Rochelle C, et al. Normal spastin gene dosage is specifically required for axon regeneration. Cell reports. 2012 Nov;2(5):1340–50.
Stone, Michelle C., et al. “Normal spastin gene dosage is specifically required for axon regeneration.Cell Reports, vol. 2, no. 5, Nov. 2012, pp. 1340–50. Epmc, doi:10.1016/j.celrep.2012.09.032.
Stone MC, Rao K, Gheres KW, Kim S, Tao J, La Rochelle C, Folker CT, Sherwood NT, Rolls MM. Normal spastin gene dosage is specifically required for axon regeneration. Cell reports. 2012 Nov;2(5):1340–1350.
Journal cover image

Published In

Cell reports

DOI

EISSN

2211-1247

ISSN

2211-1247

Publication Date

November 2012

Volume

2

Issue

5

Start / End Page

1340 / 1350

Related Subject Headings

  • Tubulin
  • RNA, Small Interfering
  • RNA Interference
  • Nerve Regeneration
  • Mutation
  • Microtubules
  • Katanin
  • Gene Dosage
  • Drosophila Proteins
  • Drosophila