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Basement membrane sliding and targeted adhesion remodels tissue boundaries during uterine-vulval attachment in Caenorhabditis elegans.

Publication ,  Journal Article
Ihara, S; Hagedorn, EJ; Morrissey, MA; Chi, Q; Motegi, F; Kramer, JM; Sherwood, DR
Published in: Nature Cell Biology
June 2011

Large gaps in basement membrane occur at sites of cell invasion and tissue remodelling in development and cancer. Though never followed directly in vivo, basement membrane dissolution or reduced synthesis have been postulated to create these gaps. Using landmark photobleaching and optical highlighting of laminin and type IV collagen, we find that a new mechanism, basement membrane sliding, underlies basement membrane gap enlargement during uterine-vulval attachment in Caenorhabditis elegans. Laser ablation and mutant analysis reveal that the invaginating vulval cells promote basement membrane movement. Further, an RNA interference and expression screen identifies the integrin INA-1/PAT-3 and VAB-19, homologue of the tumour suppressor Kank, as regulators of basement membrane opening. Both concentrate within vulval cells at the basement membrane gap boundary and halt expansion of the shifting basement membrane. Basement membrane sliding followed by targeted adhesion represents a new mechanism for creating precise basement membrane breaches that can be used by cells to break down compartment boundaries.

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Published In

Nature Cell Biology

DOI

ISSN

1476-4679

Publication Date

June 2011

Volume

13

Issue

6

Start / End Page

641 / 651

Related Subject Headings

  • Vulva
  • Uterus
  • Female
  • Developmental Biology
  • Cell Adhesion
  • Caenorhabditis elegans
  • Basement Membrane
  • Animals
  • 3101 Biochemistry and cell biology
  • 11 Medical and Health Sciences
 

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Ihara, S., Hagedorn, E. J., Morrissey, M. A., Chi, Q., Motegi, F., Kramer, J. M., & Sherwood, D. R. (2011). Basement membrane sliding and targeted adhesion remodels tissue boundaries during uterine-vulval attachment in Caenorhabditis elegans. Nature Cell Biology, 13(6), 641–651. https://doi.org/10.1038/ncb2233
Ihara, S., E. J. Hagedorn, M. A. Morrissey, Q. Chi, F. Motegi, J. M. Kramer, and D. R. Sherwood. “Basement membrane sliding and targeted adhesion remodels tissue boundaries during uterine-vulval attachment in Caenorhabditis elegans.Nature Cell Biology 13, no. 6 (June 2011): 641–51. https://doi.org/10.1038/ncb2233.
Ihara S, Hagedorn EJ, Morrissey MA, Chi Q, Motegi F, Kramer JM, et al. Basement membrane sliding and targeted adhesion remodels tissue boundaries during uterine-vulval attachment in Caenorhabditis elegans. Nature Cell Biology. 2011 Jun;13(6):641–51.
Ihara, S., et al. “Basement membrane sliding and targeted adhesion remodels tissue boundaries during uterine-vulval attachment in Caenorhabditis elegans.Nature Cell Biology, vol. 13, no. 6, June 2011, pp. 641–51. Manual, doi:10.1038/ncb2233.
Ihara S, Hagedorn EJ, Morrissey MA, Chi Q, Motegi F, Kramer JM, Sherwood DR. Basement membrane sliding and targeted adhesion remodels tissue boundaries during uterine-vulval attachment in Caenorhabditis elegans. Nature Cell Biology. 2011 Jun;13(6):641–651.

Published In

Nature Cell Biology

DOI

ISSN

1476-4679

Publication Date

June 2011

Volume

13

Issue

6

Start / End Page

641 / 651

Related Subject Headings

  • Vulva
  • Uterus
  • Female
  • Developmental Biology
  • Cell Adhesion
  • Caenorhabditis elegans
  • Basement Membrane
  • Animals
  • 3101 Biochemistry and cell biology
  • 11 Medical and Health Sciences