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A designed glycoprotein analogue of Gc-MAF exhibits native-like phagocytic activity.

Publication ,  Journal Article
Bogani, F; McConnell, E; Joshi, L; Chang, Y; Ghirlanda, G
Published in: Journal of the American Chemical Society
June 2006

Rational protein design has been successfully used to create mimics of natural proteins that retain native activity. In the present work, de novo protein engineering is explored to develop a mini-protein analogue of Gc-MAF, a glycoprotein involved in the immune system activation that has shown anticancer activity in mice. Gc-MAF is derived in vivo from vitamin D binding protein (VDBP) via enzymatic processing of its glycosaccharide to leave a single GalNAc residue located on an exposed loop. We used molecular modeling tools in conjunction with structural analysis to splice the glycosylated loop onto a stable three-helix bundle (alpha3W, PDB entry 1LQ7). The resulting 69-residue model peptide, MM1, has been successfully synthesized by solid-phase synthesis both in the aglycosylated and the glycosylated (GalNAc-MM1) form. Circular dichroism spectroscopy confirmed the expected alpha-helical secondary structure. The thermodynamic stability as evaluated from chemical and thermal denaturation is comparable with that of the scaffold protein, alpha3W, indicating that the insertion of the exogenous loop of Gc-MAF did not significantly perturb the overall structure. GalNAc-MM1 retains the macrophage stimulation activity of natural Gc-MAF; in vitro tests show an identical enhancement of Fc-receptor-mediated phagocytosis in primary macrophages. GalNAc-MM1 provides a framework for the development of mutants with increased activity that could be used in place of Gc-MAF as an immunomodulatory agent in therapy.

Duke Scholars

Published In

Journal of the American Chemical Society

DOI

EISSN

1520-5126

ISSN

0002-7863

Publication Date

June 2006

Volume

128

Issue

22

Start / End Page

7142 / 7143

Related Subject Headings

  • Vitamin D-Binding Protein
  • Phagocytosis
  • Molecular Sequence Data
  • Models, Molecular
  • Mice
  • Macrophages
  • Macrophage-Activating Factors
  • Glycosylation
  • Glycoproteins
  • General Chemistry
 

Citation

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MLA
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Bogani, F., McConnell, E., Joshi, L., Chang, Y., & Ghirlanda, G. (2006). A designed glycoprotein analogue of Gc-MAF exhibits native-like phagocytic activity. Journal of the American Chemical Society, 128(22), 7142–7143. https://doi.org/10.1021/ja0604212
Bogani, Federica, Elizabeth McConnell, Lokesh Joshi, Yung Chang, and Giovanna Ghirlanda. “A designed glycoprotein analogue of Gc-MAF exhibits native-like phagocytic activity.Journal of the American Chemical Society 128, no. 22 (June 2006): 7142–43. https://doi.org/10.1021/ja0604212.
Bogani F, McConnell E, Joshi L, Chang Y, Ghirlanda G. A designed glycoprotein analogue of Gc-MAF exhibits native-like phagocytic activity. Journal of the American Chemical Society. 2006 Jun;128(22):7142–3.
Bogani, Federica, et al. “A designed glycoprotein analogue of Gc-MAF exhibits native-like phagocytic activity.Journal of the American Chemical Society, vol. 128, no. 22, June 2006, pp. 7142–43. Epmc, doi:10.1021/ja0604212.
Bogani F, McConnell E, Joshi L, Chang Y, Ghirlanda G. A designed glycoprotein analogue of Gc-MAF exhibits native-like phagocytic activity. Journal of the American Chemical Society. 2006 Jun;128(22):7142–7143.
Journal cover image

Published In

Journal of the American Chemical Society

DOI

EISSN

1520-5126

ISSN

0002-7863

Publication Date

June 2006

Volume

128

Issue

22

Start / End Page

7142 / 7143

Related Subject Headings

  • Vitamin D-Binding Protein
  • Phagocytosis
  • Molecular Sequence Data
  • Models, Molecular
  • Mice
  • Macrophages
  • Macrophage-Activating Factors
  • Glycosylation
  • Glycoproteins
  • General Chemistry