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Mnk1 and 2 are dispensable for T cell development and activation but important for the pathogenesis of experimental autoimmune encephalomyelitis.

Publication ,  Journal Article
Gorentla, BK; Krishna, S; Shin, J; Inoue, M; Shinohara, ML; Grayson, JM; Fukunaga, R; Zhong, X-P
Published in: J Immunol
February 1, 2013

T cell development and activation are usually accompanied by expansion and production of numerous proteins that require active translation. The eukaryotic translation initiation factor 4E (eIF4E) binds to the 5' cap structure of mRNA and is critical for cap-dependent translational initiation. It has been hypothesized that MAPK-interacting kinase 1 and 2 (Mnk1/2) promote cap-dependent translation by phosphorylating eIF4E at serine 209 (S209). Pharmacologic studies using inhibitors have suggested that Mnk1/2 have important roles in T cells. However, genetic evidence supporting such conclusions is lacking. Moreover, the signaling pathways that regulate Mnk1/2 in T cells remain unclear. We demonstrate that TCR engagement activates Mnk1/2 in primary T cells. Such activation is dependent on Ras-Erk1/2 signaling and is inhibited by diacylglycerol kinases α and ζ. Mnk1/2 double deficiency in mice abolishes TCR-induced eIF4E S209 phosphorylation, indicating their absolute requirement for eIF4E S209 phosphorylation. However, Mnk1/2 double deficiency does not affect the development of conventional αβ T cells, regulatory T cells, or NKT cells. Furthermore, T cell activation, in vivo primary and memory CD8 T cell responses to microbial infection, and NKT cell cytokine production were not obviously altered by Mnk1/2 deficiency. Although Mnk1/2 deficiency causes decreased IL-17 and IFN-γ production by CD4 T cells following immunization of mice with myelin oligodendrocyte glycoprotein peptide in complete Freund's adjuvant, correlating with milder experimental autoimmune encephalomyelitis scores, it does not affect Th cell differentiation in vitro. Together, these data suggest that Mnk1/2 has a minimal role in T cell development and activation but may regulate non-T cell lineages to control Th1 and Th17 differentiation in vivo.

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Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

February 1, 2013

Volume

190

Issue

3

Start / End Page

1026 / 1037

Location

United States

Related Subject Headings

  • T-Lymphocyte Subsets
  • RNA Caps
  • Purines
  • Protein Serine-Threonine Kinases
  • Protein Processing, Post-Translational
  • Phosphorylation
  • Peptide Fragments
  • Ovalbumin
  • Natural Killer T-Cells
  • Myelin-Oligodendrocyte Glycoprotein
 

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Gorentla, B. K., Krishna, S., Shin, J., Inoue, M., Shinohara, M. L., Grayson, J. M., … Zhong, X.-P. (2013). Mnk1 and 2 are dispensable for T cell development and activation but important for the pathogenesis of experimental autoimmune encephalomyelitis. J Immunol, 190(3), 1026–1037. https://doi.org/10.4049/jimmunol.1200026
Gorentla, Balachandra K., Sruti Krishna, Jinwook Shin, Makoto Inoue, Mari L. Shinohara, Jason M. Grayson, Rikiro Fukunaga, and Xiao-Ping Zhong. “Mnk1 and 2 are dispensable for T cell development and activation but important for the pathogenesis of experimental autoimmune encephalomyelitis.J Immunol 190, no. 3 (February 1, 2013): 1026–37. https://doi.org/10.4049/jimmunol.1200026.
Gorentla BK, Krishna S, Shin J, Inoue M, Shinohara ML, Grayson JM, et al. Mnk1 and 2 are dispensable for T cell development and activation but important for the pathogenesis of experimental autoimmune encephalomyelitis. J Immunol. 2013 Feb 1;190(3):1026–37.
Gorentla, Balachandra K., et al. “Mnk1 and 2 are dispensable for T cell development and activation but important for the pathogenesis of experimental autoimmune encephalomyelitis.J Immunol, vol. 190, no. 3, Feb. 2013, pp. 1026–37. Pubmed, doi:10.4049/jimmunol.1200026.
Gorentla BK, Krishna S, Shin J, Inoue M, Shinohara ML, Grayson JM, Fukunaga R, Zhong X-P. Mnk1 and 2 are dispensable for T cell development and activation but important for the pathogenesis of experimental autoimmune encephalomyelitis. J Immunol. 2013 Feb 1;190(3):1026–1037.

Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

February 1, 2013

Volume

190

Issue

3

Start / End Page

1026 / 1037

Location

United States

Related Subject Headings

  • T-Lymphocyte Subsets
  • RNA Caps
  • Purines
  • Protein Serine-Threonine Kinases
  • Protein Processing, Post-Translational
  • Phosphorylation
  • Peptide Fragments
  • Ovalbumin
  • Natural Killer T-Cells
  • Myelin-Oligodendrocyte Glycoprotein