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Immortalized hypothalamic gonadotropin-releasing hormone neurons.

Publication ,  Journal Article
Mellon, PL; Wetsel, WC; Windle, JJ; Valença, MM; Goldsmith, PC; Whyte, DB; Eraly, SA; Negro-Vilar, A; Weiner, RI
Published in: Ciba Found Symp
1992

The neuroendocrine hypothalamus has been intensively studied using whole animals and tissue slices. However, it has been difficult to approach questions at the molecular and cellular level. By targeting expression of the oncogene product, simian virus 40 T antigen, in transgenic mice using the regulatory domain of the rat gonadotropin-releasing hormone (GnRH) gene, we have produced specific hypothalamic tumours. These tumours have been cultured to produce clonal cell lines (GT-1 cells) that express T antigen, GnRH and many other neuronal markers, but do not express other hypothalamic hormones. These immortal cell lines have a distinctive neuronal phenotype, process the GnRH peptide accurately and secrete GnRH in a pulsatile pattern. Thus, by targeting oncogenesis to a defined population of neurons using the regulatory region of a gene that is expressed late in differentiation of that cell lineage, we have succeeded in immortalizing hypothalamic GnRH neurons. The GT-1 cell lines are an excellent model for future molecular, cell biological, physiological and biochemical investigations into the mechanisms involved in regulation of GnRH and the characteristics of an isolated central nervous system neuron. Their derivation demonstrates the utility of targeting tumorigenesis to specific differentiated neurons of the central nervous system in transgenic mice.

Duke Scholars

Published In

Ciba Found Symp

DOI

ISSN

0300-5208

Publication Date

1992

Volume

168

Start / End Page

104 / 117

Location

Netherlands

Related Subject Headings

  • Simian virus 40
  • Oncogenes
  • Neuropeptides
  • Neurons
  • Mice, Transgenic
  • Mice
  • Male
  • Infertility
  • Hypothalamus
  • Gonadotropin-Releasing Hormone
 

Citation

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ICMJE
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Mellon, P. L., Wetsel, W. C., Windle, J. J., Valença, M. M., Goldsmith, P. C., Whyte, D. B., … Weiner, R. I. (1992). Immortalized hypothalamic gonadotropin-releasing hormone neurons. Ciba Found Symp, 168, 104–117. https://doi.org/10.1002/9780470514283.ch8
Mellon, P. L., W. C. Wetsel, J. J. Windle, M. M. Valença, P. C. Goldsmith, D. B. Whyte, S. A. Eraly, A. Negro-Vilar, and R. I. Weiner. “Immortalized hypothalamic gonadotropin-releasing hormone neurons.Ciba Found Symp 168 (1992): 104–17. https://doi.org/10.1002/9780470514283.ch8.
Mellon PL, Wetsel WC, Windle JJ, Valença MM, Goldsmith PC, Whyte DB, et al. Immortalized hypothalamic gonadotropin-releasing hormone neurons. Ciba Found Symp. 1992;168:104–17.
Mellon, P. L., et al. “Immortalized hypothalamic gonadotropin-releasing hormone neurons.Ciba Found Symp, vol. 168, 1992, pp. 104–17. Pubmed, doi:10.1002/9780470514283.ch8.
Mellon PL, Wetsel WC, Windle JJ, Valença MM, Goldsmith PC, Whyte DB, Eraly SA, Negro-Vilar A, Weiner RI. Immortalized hypothalamic gonadotropin-releasing hormone neurons. Ciba Found Symp. 1992;168:104–117.

Published In

Ciba Found Symp

DOI

ISSN

0300-5208

Publication Date

1992

Volume

168

Start / End Page

104 / 117

Location

Netherlands

Related Subject Headings

  • Simian virus 40
  • Oncogenes
  • Neuropeptides
  • Neurons
  • Mice, Transgenic
  • Mice
  • Male
  • Infertility
  • Hypothalamus
  • Gonadotropin-Releasing Hormone