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Human natural killer cells analyzed by B73.1, a monoclonal antibody blocking Fc receptor functions. I. Characterization of the lymphocyte subset reactive with B73.1.

Publication ,  Journal Article
Perussia, B; Starr, S; Abraham, S; Fanning, V; Trinchieri, G
Published in: J Immunol
May 1983

We describe the production of the monoclonal antibody B73.1, reacting with a subset of human lymphocytes and, in about one-half of the donors, with neutrophilic polymorphonuclear leukocytes. In the peripheral blood from normal adult donors, 14.6 +/- 8.5% of the lymphocytes react with B73.1 antibody. The B73.1(+) lymphocyte subset does not bear markers of typical T or B cells and corresponds to the lymphocyte subset containing antibody-dependent killer (K) and natural killer (NK) cells. We demonstrate that: a) virtually all lymphocytes with K/NK cytotoxic activity are found in the lymphocyte subpopulation bearing the B73.1-defined antigen; b) the B73.1(+) lymphocyte subset bears the combination of antigens known to be present on K/NK cells; and c) there is a positive correlation between the level of cytotoxicity and the actual number of B73.1(+) lymphocytes in individual donors. We also report the distribution of B73.1(+) lymphocytes according to donor age and tissue types. The use of the B73.1 antibody in quantitating the actual number of K/NK cells and in performing functional studies on spontaneous cytotoxicity is discussed.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

May 1983

Volume

130

Issue

5

Start / End Page

2133 / 2141

Location

United States

Related Subject Headings

  • Receptors, Fc
  • Neutrophils
  • Mice
  • Killer Cells, Natural
  • Infant, Newborn
  • Infant
  • Immunology
  • Humans
  • Fetal Blood
  • Cytotoxicity, Immunologic
 

Published In

J Immunol

ISSN

0022-1767

Publication Date

May 1983

Volume

130

Issue

5

Start / End Page

2133 / 2141

Location

United States

Related Subject Headings

  • Receptors, Fc
  • Neutrophils
  • Mice
  • Killer Cells, Natural
  • Infant, Newborn
  • Infant
  • Immunology
  • Humans
  • Fetal Blood
  • Cytotoxicity, Immunologic