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The sequence of the reovirus serotype 3 L3 genome segment which encodes the major core protein lambda 1.

Publication ,  Journal Article
Bartlett, JA; Joklik, WK
Published in: Virology
November 1988

We present the sequence of reovirus serotype 3 (strain Dearing) genome segment L3 which encodes protein lambda 1, one of the two major components of the core shell. The genome segment is 3896 nucleotides long, with 5'- and 3'-noncoding regions of 13 and 181 nucleotides, respectively. Protein lambda 1 is 1233 amino acids long. It is a slightly acidic protein, with a predicted alpha-helix and beta-sheet content of 23.6 and 28.3%, respectively. Its rather low predicted alpha-helix contact is consistent with its being a structural protein. The 123 amino acid long region at its amino terminus is very hydrophilic and contains three alpha-helical regions, one being 26 amino acids long. Protein lambda 1 contains two functional motifs. The first is a nucleotide binding site -TKGKSSG- starting at residue 8, the other is a "zinc finger" motif centered around amino acid residue 194. This suggests that protein lambda 1 functions during the transcription of either dsRNA into plus strands or of plus strands into minus strands, or during both. It displays no significant sequence similarity to any protein sequence in the GenBank data base.

Duke Scholars

Published In

Virology

DOI

ISSN

0042-6822

Publication Date

November 1988

Volume

167

Issue

1

Start / End Page

31 / 37

Location

United States

Related Subject Headings

  • Virology
  • Viral Core Proteins
  • Reoviridae
  • Protein Conformation
  • Molecular Sequence Data
  • Mammalian orthoreovirus 3
  • DNA, Viral
  • Codon
  • Cloning, Molecular
  • Base Sequence
 

Citation

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MLA
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Bartlett, J. A., & Joklik, W. K. (1988). The sequence of the reovirus serotype 3 L3 genome segment which encodes the major core protein lambda 1. Virology, 167(1), 31–37. https://doi.org/10.1016/0042-6822(88)90051-7
Bartlett, J. A., and W. K. Joklik. “The sequence of the reovirus serotype 3 L3 genome segment which encodes the major core protein lambda 1.Virology 167, no. 1 (November 1988): 31–37. https://doi.org/10.1016/0042-6822(88)90051-7.
Bartlett, J. A., and W. K. Joklik. “The sequence of the reovirus serotype 3 L3 genome segment which encodes the major core protein lambda 1.Virology, vol. 167, no. 1, Nov. 1988, pp. 31–37. Pubmed, doi:10.1016/0042-6822(88)90051-7.
Journal cover image

Published In

Virology

DOI

ISSN

0042-6822

Publication Date

November 1988

Volume

167

Issue

1

Start / End Page

31 / 37

Location

United States

Related Subject Headings

  • Virology
  • Viral Core Proteins
  • Reoviridae
  • Protein Conformation
  • Molecular Sequence Data
  • Mammalian orthoreovirus 3
  • DNA, Viral
  • Codon
  • Cloning, Molecular
  • Base Sequence