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Crystallization of mutant lysozymes from bacteriophage T4

Publication ,  Journal Article
Brennan, RG; Wozniak, J; Faber, R; Matthews, BW
Published in: Journal of Crystal Growth
July 2, 1988

The crystallization properties of a large number of T4 lysozyme mutant proteins have been analyzed. Approximately 80% of the mutant proteins crystallize under conditions very similar to those used for the wild-type protein, regardless of the type of amino acid substitution or its location. Of the mutants that crystallize all but two, A146F (tetragonal) and M6I (orthorhombic), are isomorphous with wild-type lysozyme (trigonal). The two nonisomorphous crystals are obtained in cases where internal residues are changed. Substitution of a large side chain for a small one, as in A146F, appears to exceed a critical internal packing volume above which nonisomorphism is induced. The nonisomorphism of M6I may be due to structural changes induced by the introduction of a β-branched side chain. © 1988.

Duke Scholars

Published In

Journal of Crystal Growth

DOI

ISSN

0022-0248

Publication Date

July 2, 1988

Volume

90

Issue

1-3

Start / End Page

160 / 167

Related Subject Headings

  • Applied Physics
  • 4016 Materials engineering
  • 3403 Macromolecular and materials chemistry
  • 0912 Materials Engineering
  • 0306 Physical Chemistry (incl. Structural)
  • 0303 Macromolecular and Materials Chemistry
 

Citation

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Brennan, R. G., Wozniak, J., Faber, R., & Matthews, B. W. (1988). Crystallization of mutant lysozymes from bacteriophage T4. Journal of Crystal Growth, 90(1–3), 160–167. https://doi.org/10.1016/0022-0248(88)90311-9
Brennan, R. G., J. Wozniak, R. Faber, and B. W. Matthews. “Crystallization of mutant lysozymes from bacteriophage T4.” Journal of Crystal Growth 90, no. 1–3 (July 2, 1988): 160–67. https://doi.org/10.1016/0022-0248(88)90311-9.
Brennan RG, Wozniak J, Faber R, Matthews BW. Crystallization of mutant lysozymes from bacteriophage T4. Journal of Crystal Growth. 1988 Jul 2;90(1–3):160–7.
Brennan, R. G., et al. “Crystallization of mutant lysozymes from bacteriophage T4.” Journal of Crystal Growth, vol. 90, no. 1–3, July 1988, pp. 160–67. Scopus, doi:10.1016/0022-0248(88)90311-9.
Brennan RG, Wozniak J, Faber R, Matthews BW. Crystallization of mutant lysozymes from bacteriophage T4. Journal of Crystal Growth. 1988 Jul 2;90(1–3):160–167.
Journal cover image

Published In

Journal of Crystal Growth

DOI

ISSN

0022-0248

Publication Date

July 2, 1988

Volume

90

Issue

1-3

Start / End Page

160 / 167

Related Subject Headings

  • Applied Physics
  • 4016 Materials engineering
  • 3403 Macromolecular and materials chemistry
  • 0912 Materials Engineering
  • 0306 Physical Chemistry (incl. Structural)
  • 0303 Macromolecular and Materials Chemistry