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Comparison of renin-specific IgG and antibody fragments in studies of blood pressure regulation

Publication ,  Journal Article
Dzau, VJ; Kopelman, RI; Barger, AC; Haber, R
Published in: American Journal of Physiology - Heart and Circulatory Physiology
January 1, 1984

The use of antirenin antibody and its fragments as specific tools for the analysis of the role of renin in blood pressure regulation is assessed in this study. Specific immunoglobulin G (IgG) or its antibody fragments (Fab) raised against purified canine renal renin were employed. These agents had no effect on blood pressure in the sodium-replete unanesthetized dog but induced systemic vasodepressor response (16.4 ± 2 mmHg mean pressure) and suppression of plasma renin activity in sodium-deplete animals. Renin-specific IgG or Fab restored mean systemic pressure of acute renovascular hypertensive dogs to normotensive levels (132 ± 2 to 90 ± 5 mmHg) associated with inhibition of plasma renin activity (11.2 ± 2 to 1.4 ± 0.7 ng angiotensin I·ml -1 ·h -1 ). A linear relationship was demonstrated between the reduction in mean systemic pressure and fall in plasma renin activity (r = 0.87, P < 0.005). The onset of action of both agents occurred within minutes. However, the peak effect of Fab was observed within 1-2 min, while that of IgG was delayed by approximately 20 min. The duration of action of Fab was short lived (about 30 min) whereas that for IgG was prolonged (up to 24 h). The results demonstrate the usefulness of renin-specific IgG and Fab as tools in physiological studies.

Duke Scholars

Published In

American Journal of Physiology - Heart and Circulatory Physiology

ISSN

0363-6135

Publication Date

January 1, 1984

Volume

15

Issue

3

Related Subject Headings

  • Cardiovascular System & Hematology
  • 1116 Medical Physiology
  • 0606 Physiology
 

Citation

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Dzau, V. J., Kopelman, R. I., Barger, A. C., & Haber, R. (1984). Comparison of renin-specific IgG and antibody fragments in studies of blood pressure regulation. American Journal of Physiology - Heart and Circulatory Physiology, 15(3).
Dzau, V. J., R. I. Kopelman, A. C. Barger, and R. Haber. “Comparison of renin-specific IgG and antibody fragments in studies of blood pressure regulation.” American Journal of Physiology - Heart and Circulatory Physiology 15, no. 3 (January 1, 1984).
Dzau VJ, Kopelman RI, Barger AC, Haber R. Comparison of renin-specific IgG and antibody fragments in studies of blood pressure regulation. American Journal of Physiology - Heart and Circulatory Physiology. 1984 Jan 1;15(3).
Dzau, V. J., et al. “Comparison of renin-specific IgG and antibody fragments in studies of blood pressure regulation.” American Journal of Physiology - Heart and Circulatory Physiology, vol. 15, no. 3, Jan. 1984.
Dzau VJ, Kopelman RI, Barger AC, Haber R. Comparison of renin-specific IgG and antibody fragments in studies of blood pressure regulation. American Journal of Physiology - Heart and Circulatory Physiology. 1984 Jan 1;15(3).

Published In

American Journal of Physiology - Heart and Circulatory Physiology

ISSN

0363-6135

Publication Date

January 1, 1984

Volume

15

Issue

3

Related Subject Headings

  • Cardiovascular System & Hematology
  • 1116 Medical Physiology
  • 0606 Physiology