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The production and characterization of murine monoclonal antibodies to human Gadd45 raised against a recombinant protein.

Publication ,  Journal Article
Kilpatrick, KE; Carrier, F; Smith, ML; Chen, CY; Lee, AJ; Rusnak, DW; Kastan, MB; Fornace, AJ; Champion, BR; Gilmer, TM
Published in: Hybridoma
August 1995

The production of two different murine monoclonal antibodies to human Gadd45, a protein that is induced in response to DNA damage, is reported. Antibodies were generated in a SJL mouse using a recombinant form of the human Gadd45 protein. Monoclonal antibody 4TCYA1, which recognizes the denatured form of human Gadd45 in Western blots, was selected based upon the recognition of Gadd45 induced by functional p53 in the human myeloid leukemia cell line, ML-1. A second monoclonal antibody, designated 30T.14, immunoprecipitates native human Gadd45 in lysates produced from RKO cells, a colorectal carcinoma cell line that expresses relatively high basal levels of Gadd45, as well as from cell lysates made from ML-1 cells after exposure to ionizing irradiation (IR). Since 4TCYA1 fails to immunoprecipitate Gadd45, and 30T.14 fails to bind to IR-induced Gadd45 in immunoblotting, these two monoclonal antibodies probably recognize different epitopes.

Duke Scholars

Published In

Hybridoma

DOI

ISSN

0272-457X

Publication Date

August 1995

Volume

14

Issue

4

Start / End Page

355 / 359

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Recombinant Proteins
  • Proteins
  • Mice, Inbred Strains
  • Mice
  • Intracellular Signaling Peptides and Proteins
  • Hybridomas
  • Humans
  • GADD45 Proteins
  • Female
 

Citation

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Chicago
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Kilpatrick, K. E., Carrier, F., Smith, M. L., Chen, C. Y., Lee, A. J., Rusnak, D. W., … Gilmer, T. M. (1995). The production and characterization of murine monoclonal antibodies to human Gadd45 raised against a recombinant protein. Hybridoma, 14(4), 355–359. https://doi.org/10.1089/hyb.1995.14.355
Kilpatrick, K. E., F. Carrier, M. L. Smith, C. Y. Chen, A. J. Lee, D. W. Rusnak, M. B. Kastan, A. J. Fornace, B. R. Champion, and T. M. Gilmer. “The production and characterization of murine monoclonal antibodies to human Gadd45 raised against a recombinant protein.Hybridoma 14, no. 4 (August 1995): 355–59. https://doi.org/10.1089/hyb.1995.14.355.
Kilpatrick KE, Carrier F, Smith ML, Chen CY, Lee AJ, Rusnak DW, et al. The production and characterization of murine monoclonal antibodies to human Gadd45 raised against a recombinant protein. Hybridoma. 1995 Aug;14(4):355–9.
Kilpatrick, K. E., et al. “The production and characterization of murine monoclonal antibodies to human Gadd45 raised against a recombinant protein.Hybridoma, vol. 14, no. 4, Aug. 1995, pp. 355–59. Pubmed, doi:10.1089/hyb.1995.14.355.
Kilpatrick KE, Carrier F, Smith ML, Chen CY, Lee AJ, Rusnak DW, Kastan MB, Fornace AJ, Champion BR, Gilmer TM. The production and characterization of murine monoclonal antibodies to human Gadd45 raised against a recombinant protein. Hybridoma. 1995 Aug;14(4):355–359.

Published In

Hybridoma

DOI

ISSN

0272-457X

Publication Date

August 1995

Volume

14

Issue

4

Start / End Page

355 / 359

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Recombinant Proteins
  • Proteins
  • Mice, Inbred Strains
  • Mice
  • Intracellular Signaling Peptides and Proteins
  • Hybridomas
  • Humans
  • GADD45 Proteins
  • Female