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Flow cytometric identification of intracellular antigens: detection of minimal residual leukemia.

Publication ,  Journal Article
Gore, S; Kastan, M; Civin, C
Published in: Bone Marrow Transplant
July 1990

Recent advances in preparation of cells for flow cytometric analysis have enabled the sensitive detection of intracellular antigens. We have examined the utility of two color flow cytometry for the detection of minimal residual T cell acute lymphoblastic leukemia (T-ALL) using a combination of expression of the pan-T cell marker CD5 and intranuclear terminal deoxynucleotidyl transferase (TdT). CD5+TdT+ cells can be sensitively detected above background in remission bone marrows (0.03% sensitivity). Possible extension of this technique to the detection of minimal residual B-lineage ALL, acute myelogenous leukemia (AML), and non-hematologic malignancies is discussed.

Duke Scholars

Published In

Bone Marrow Transplant

ISSN

0268-3369

Publication Date

July 1990

Volume

6 Suppl 1

Start / End Page

39 / 41

Location

England

Related Subject Headings

  • Leukemia-Lymphoma, Adult T-Cell
  • Leukemia
  • Immunology
  • Humans
  • Flow Cytometry
  • DNA Nucleotidylexotransferase
  • CD5 Antigens
  • Bone Marrow
  • Biomarkers, Tumor
  • Antigens, Neoplasm
 

Citation

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ICMJE
MLA
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Gore, S., Kastan, M., & Civin, C. (1990). Flow cytometric identification of intracellular antigens: detection of minimal residual leukemia. Bone Marrow Transplant, 6 Suppl 1, 39–41.
Gore, S., M. Kastan, and C. Civin. “Flow cytometric identification of intracellular antigens: detection of minimal residual leukemia.Bone Marrow Transplant 6 Suppl 1 (July 1990): 39–41.
Gore S, Kastan M, Civin C. Flow cytometric identification of intracellular antigens: detection of minimal residual leukemia. Bone Marrow Transplant. 1990 Jul;6 Suppl 1:39–41.
Gore, S., et al. “Flow cytometric identification of intracellular antigens: detection of minimal residual leukemia.Bone Marrow Transplant, vol. 6 Suppl 1, July 1990, pp. 39–41.
Gore S, Kastan M, Civin C. Flow cytometric identification of intracellular antigens: detection of minimal residual leukemia. Bone Marrow Transplant. 1990 Jul;6 Suppl 1:39–41.

Published In

Bone Marrow Transplant

ISSN

0268-3369

Publication Date

July 1990

Volume

6 Suppl 1

Start / End Page

39 / 41

Location

England

Related Subject Headings

  • Leukemia-Lymphoma, Adult T-Cell
  • Leukemia
  • Immunology
  • Humans
  • Flow Cytometry
  • DNA Nucleotidylexotransferase
  • CD5 Antigens
  • Bone Marrow
  • Biomarkers, Tumor
  • Antigens, Neoplasm