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Detection of minimal residual T cell acute lymphoblastic leukemia by flow cytometry.

Publication ,  Journal Article
Gore, SD; Kastan, MB; Goodman, SN; Civin, CI
Published in: J Immunol Methods
September 14, 1990

We have developed a flow cytometric assay for the determination of cellular expression of terminal deoxynucleotidyl transferase (TdT) and applied this to the detection of minimal residual T cell acute lymphoblastic leukemia (T-ALL). The flow cytometric assay for TdT demonstrated requisite specificity: TdT was localized to the nucleus, and was detected in MOLT3 T lymphoblasts, clinical T-ALL samples, and normal bone marrow B lymphoid precursors, but in neither the KG1a myeloid leukemia cell line nor normal myeloid cells. Co-expression of TdT and the pan T cell marker CD5 was used to quantify T lymphoblasts. 0.25 +/- 0.13% of normal adult bone marrow CD5+ cells were TdT+; these may represent early T lymphoid precursors. When admixed with normal bone marrow, CD5+TdT+ leukemic cells could be detected above background levels at an added concentration of 0.035% (95% confidence interval 0.028-0.43%). Long term follow-up of a large number of patients will be required to determine the clinical significance of a minimal burden of leukemic cells.

Duke Scholars

Published In

J Immunol Methods

DOI

ISSN

0022-1759

Publication Date

September 14, 1990

Volume

132

Issue

2

Start / End Page

275 / 286

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Time Factors
  • T-Lymphocytes
  • Neprilysin
  • Leukemia-Lymphoma, Adult T-Cell
  • Immunology
  • Humans
  • Flow Cytometry
  • DNA Nucleotidylexotransferase
  • Bone Marrow Cells
 

Citation

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Gore, S. D., Kastan, M. B., Goodman, S. N., & Civin, C. I. (1990). Detection of minimal residual T cell acute lymphoblastic leukemia by flow cytometry. J Immunol Methods, 132(2), 275–286. https://doi.org/10.1016/0022-1759(90)90039-x
Gore, S. D., M. B. Kastan, S. N. Goodman, and C. I. Civin. “Detection of minimal residual T cell acute lymphoblastic leukemia by flow cytometry.J Immunol Methods 132, no. 2 (September 14, 1990): 275–86. https://doi.org/10.1016/0022-1759(90)90039-x.
Gore SD, Kastan MB, Goodman SN, Civin CI. Detection of minimal residual T cell acute lymphoblastic leukemia by flow cytometry. J Immunol Methods. 1990 Sep 14;132(2):275–86.
Gore, S. D., et al. “Detection of minimal residual T cell acute lymphoblastic leukemia by flow cytometry.J Immunol Methods, vol. 132, no. 2, Sept. 1990, pp. 275–86. Pubmed, doi:10.1016/0022-1759(90)90039-x.
Gore SD, Kastan MB, Goodman SN, Civin CI. Detection of minimal residual T cell acute lymphoblastic leukemia by flow cytometry. J Immunol Methods. 1990 Sep 14;132(2):275–286.
Journal cover image

Published In

J Immunol Methods

DOI

ISSN

0022-1759

Publication Date

September 14, 1990

Volume

132

Issue

2

Start / End Page

275 / 286

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Time Factors
  • T-Lymphocytes
  • Neprilysin
  • Leukemia-Lymphoma, Adult T-Cell
  • Immunology
  • Humans
  • Flow Cytometry
  • DNA Nucleotidylexotransferase
  • Bone Marrow Cells