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Methylation of deoxycytidine incorporated by excision-repair synthesis of DNA.

Publication ,  Journal Article
Kastan, MB; Gowans, BJ; Lieberman, MW
Published in: Cell
September 1982

Methylation of deoxycytidine incorporated by DNA excision-repair was studied in human diploid fibroblasts following damage with ultraviolet radiation, N-methyl-N-nitrosourea, or N-acetoxy-2-acetylaminofluorene. In confluent, nondividing cells, methylation in repair patches induced by all three agents is slow and incomplete. Whereas after DNA replication in logarithmic-phase cultures a steady state level of 3.4% 5-methylcytosine is reached in less than 2 hr after cells are labeled with 6- 3H-deoxycytidine, following ultraviolet-stimulated repair synthesis in confluent cells it takes about 3 days to reach a level of approximately 2.0% 5-methylcytosine in the repair patch. In cells from cultures in logarithmic-phase growth, 5-methylcytosine formation in ultraviolet-induced repair patches occurs faster and to a greater extent, reaching a level of approximately 2.7% in 10-20 hr. Preexisting hypomethylated repair patches in confluent cells are methylated further when the cells are stimulated to divide; however, the repair patch may still not be fully methylated before cell division occurs. Thus DNA damage and repair may lead to heritable loss of methylation at some sites.

Duke Scholars

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

September 1982

Volume

30

Issue

2

Start / End Page

509 / 516

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Methylnitrosourea
  • Methylation
  • Humans
  • Fibroblasts
  • Developmental Biology
  • Deoxycytidine
  • DNA Replication
  • DNA Repair
  • DNA
 

Citation

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MLA
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Kastan, M. B., Gowans, B. J., & Lieberman, M. W. (1982). Methylation of deoxycytidine incorporated by excision-repair synthesis of DNA. Cell, 30(2), 509–516. https://doi.org/10.1016/0092-8674(82)90248-3
Kastan, M. B., B. J. Gowans, and M. W. Lieberman. “Methylation of deoxycytidine incorporated by excision-repair synthesis of DNA.Cell 30, no. 2 (September 1982): 509–16. https://doi.org/10.1016/0092-8674(82)90248-3.
Kastan MB, Gowans BJ, Lieberman MW. Methylation of deoxycytidine incorporated by excision-repair synthesis of DNA. Cell. 1982 Sep;30(2):509–16.
Kastan, M. B., et al. “Methylation of deoxycytidine incorporated by excision-repair synthesis of DNA.Cell, vol. 30, no. 2, Sept. 1982, pp. 509–16. Pubmed, doi:10.1016/0092-8674(82)90248-3.
Kastan MB, Gowans BJ, Lieberman MW. Methylation of deoxycytidine incorporated by excision-repair synthesis of DNA. Cell. 1982 Sep;30(2):509–516.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

September 1982

Volume

30

Issue

2

Start / End Page

509 / 516

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Methylnitrosourea
  • Methylation
  • Humans
  • Fibroblasts
  • Developmental Biology
  • Deoxycytidine
  • DNA Replication
  • DNA Repair
  • DNA