Scholars@Duke publication: RNA sequence and transcriptional properties of the 3' end of the Newcastle disease virus genome.

RNA sequence and transcriptional properties of the 3' end of the Newcastle disease virus genome.

Publication , Journal Article

Kurilla, MG; Stone, HO; Keene, JD

Published in: Virology

September 1985

The 3' end of the genomic RNA of Newcastle disease virus (NDV) has been sequenced and the leader RNA defined. Using hybridization to a 3'-end-labeled genome, leader RNA species from in vitro transcription reactions and from infected cell extracts were found to be 47 and 53 nucleotides long. In addition, the start site of the 3'-proximal mRNA was determined by sequence analysis of in vitro [beta-82P]GTP-labeled transcription products. The genomic sequence extending beyond the leader region demonstrated an open reading frame for at least 42 amino acids and probably represents the amino terminus of the nucleocapsid protein (NP). The terminal 8 nucleotides of the NDV genome were identical to those of measles virus and Sendai virus while the sequence of the distal half of the leader region was more similar to that of vesicular stomatitis virus. These data argue for strong evolutionary relatedness between the paramyxovirus and rhabdovirus groups.

Duke Scholars

Author Jack Donald Keene Molecular Genetics and Microbiology

Published In

Virology

DOI

10.1016/0042-6822(85)90154-0

ISSN

0042-6822

Publication Date

September 1985

Volume

145

Issue

Start / End Page

203 / 212

Location

United States

Related Subject Headings

Virology
Transcription, Genetic
RNA, Viral
Phosphorus Radioisotopes
Oligoribonucleotides
Nucleic Acid Hybridization
Newcastle disease virus
Genes, Viral
Chick Embryo
Base Sequence

Citation

APA

Chicago

ICMJE

MLA

NLM

Kurilla, M. G., Stone, H. O., & Keene, J. D. (1985). RNA sequence and transcriptional properties of the 3' end of the Newcastle disease virus genome. Virology, 145(2), 203–212. https://doi.org/10.1016/0042-6822(85)90154-0

Kurilla, M. G., H. O. Stone, and J. D. Keene. “RNA sequence and transcriptional properties of the 3' end of the Newcastle disease virus genome.” Virology 145, no. 2 (September 1985): 203–12. https://doi.org/10.1016/0042-6822(85)90154-0.

Kurilla MG, Stone HO, Keene JD. RNA sequence and transcriptional properties of the 3' end of the Newcastle disease virus genome. Virology. 1985 Sep;145(2):203–12.

Kurilla, M. G., et al. “RNA sequence and transcriptional properties of the 3' end of the Newcastle disease virus genome.” Virology, vol. 145, no. 2, Sept. 1985, pp. 203–12. Pubmed, doi:10.1016/0042-6822(85)90154-0.

Kurilla MG, Stone HO, Keene JD. RNA sequence and transcriptional properties of the 3' end of the Newcastle disease virus genome. Virology. 1985 Sep;145(2):203–212.