Skip to main content

Multiple cell-type-specific elements regulate Myc protein stability.

Publication ,  Journal Article
Herbst, A; Salghetti, SE; Kim, SY; Tansey, WP
Published in: Oncogene
May 6, 2004

Myc is a highly unstable transcription factor that is destroyed by ubiquitin (Ub)-mediated proteolysis. We have previously identified an amino-terminal 'degron' within Myc that signals its destruction; this degron spans the transcriptional activation domain of Myc, and includes two highly conserved regions called Myc boxes I and II. We now report the identification of a second element--the D-element--which is also required for Myc proteolysis. The centrally located D-element is distinct from the PEST domain in Myc, but includes Myc box III, a third highly conserved region with no previously known function. We show that deletion of the D-element stabilizes the Myc protein without affecting its ubiquitylation, and report that the D-element and the degron act in a cell-type-specific manner to direct Myc proteolysis. These data thus demonstrate that Myc stability is regulated at both the ubiquitylation and postubiquitylation levels, and reveal that substrates of the Ub-proteasome system can be targeted for destruction differently in different cell types.

Duke Scholars

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

May 6, 2004

Volume

23

Issue

21

Start / End Page

3863 / 3871

Location

England

Related Subject Headings

  • Ubiquitin
  • Rats
  • Proto-Oncogene Proteins c-myc
  • Proteasome Endopeptidase Complex
  • Oncology & Carcinogenesis
  • Multienzyme Complexes
  • Molecular Sequence Data
  • Humans
  • Cysteine Endopeptidases
  • Conserved Sequence
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Herbst, A., Salghetti, S. E., Kim, S. Y., & Tansey, W. P. (2004). Multiple cell-type-specific elements regulate Myc protein stability. Oncogene, 23(21), 3863–3871. https://doi.org/10.1038/sj.onc.1207492
Herbst, Andreas, Simone E. Salghetti, So Young Kim, and William P. Tansey. “Multiple cell-type-specific elements regulate Myc protein stability.Oncogene 23, no. 21 (May 6, 2004): 3863–71. https://doi.org/10.1038/sj.onc.1207492.
Herbst A, Salghetti SE, Kim SY, Tansey WP. Multiple cell-type-specific elements regulate Myc protein stability. Oncogene. 2004 May 6;23(21):3863–71.
Herbst, Andreas, et al. “Multiple cell-type-specific elements regulate Myc protein stability.Oncogene, vol. 23, no. 21, May 2004, pp. 3863–71. Pubmed, doi:10.1038/sj.onc.1207492.
Herbst A, Salghetti SE, Kim SY, Tansey WP. Multiple cell-type-specific elements regulate Myc protein stability. Oncogene. 2004 May 6;23(21):3863–3871.

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

May 6, 2004

Volume

23

Issue

21

Start / End Page

3863 / 3871

Location

England

Related Subject Headings

  • Ubiquitin
  • Rats
  • Proto-Oncogene Proteins c-myc
  • Proteasome Endopeptidase Complex
  • Oncology & Carcinogenesis
  • Multienzyme Complexes
  • Molecular Sequence Data
  • Humans
  • Cysteine Endopeptidases
  • Conserved Sequence