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Protein isoforms encoded by the pX region of human T-cell leukemia/lymphotropic virus type I.

Publication ,  Journal Article
Koralnik, IJ; Gessain, A; Klotman, ME; Lo Monico, A; Berneman, ZN; Franchini, G
Published in: Proc Natl Acad Sci U S A
September 15, 1992

The pX region of the human T-cell leukemia/lymphotropic virus type I (HTLV-I) contains at least four open reading frames (orfI-orfIV). orf III and orf IV encode the regulatory HTLV-I proteins Rex and Tax, which together modulate viral expression, and the p21rex protein of unknown function. By using the reverse transcriptase and polymerase chain reaction techniques on the RNA of an HTLV-I-infected cell culture, we uncovered the existence of alternatively spliced mRNAs generated through the use of three splice acceptor sites. These mRNAs encoded protein isoforms derived from the HTLV-I orf I (p12I) and orf II (p13II and p30II). An additional acceptor splice site, used in the processing of the env and tax/rex mRNAs and a singly spliced mRNA for the p21rex protein, was also identified. All of these HTLV-I mRNAs were also detected in freshly isolated cells from HTLV-I-infected individuals. Thus HTLV-I, like the human immunodeficiency virus type 1, has developed fine posttranscriptional mechanisms to increase the complexity of its genome.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

September 15, 1992

Volume

89

Issue

18

Start / End Page

8813 / 8817

Location

United States

Related Subject Headings

  • RNA Splicing
  • Polymerase Chain Reaction
  • Open Reading Frames
  • Oligodeoxyribonucleotides
  • Molecular Sequence Data
  • Humans
  • Human T-lymphotropic virus 1
  • HTLV-I Infections
  • Genes, pX
  • Genes, Overlapping
 

Citation

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ICMJE
MLA
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Koralnik, I. J., Gessain, A., Klotman, M. E., Lo Monico, A., Berneman, Z. N., & Franchini, G. (1992). Protein isoforms encoded by the pX region of human T-cell leukemia/lymphotropic virus type I. Proc Natl Acad Sci U S A, 89(18), 8813–8817. https://doi.org/10.1073/pnas.89.18.8813
Koralnik, I. J., A. Gessain, M. E. Klotman, A. Lo Monico, Z. N. Berneman, and G. Franchini. “Protein isoforms encoded by the pX region of human T-cell leukemia/lymphotropic virus type I.Proc Natl Acad Sci U S A 89, no. 18 (September 15, 1992): 8813–17. https://doi.org/10.1073/pnas.89.18.8813.
Koralnik IJ, Gessain A, Klotman ME, Lo Monico A, Berneman ZN, Franchini G. Protein isoforms encoded by the pX region of human T-cell leukemia/lymphotropic virus type I. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8813–7.
Koralnik, I. J., et al. “Protein isoforms encoded by the pX region of human T-cell leukemia/lymphotropic virus type I.Proc Natl Acad Sci U S A, vol. 89, no. 18, Sept. 1992, pp. 8813–17. Pubmed, doi:10.1073/pnas.89.18.8813.
Koralnik IJ, Gessain A, Klotman ME, Lo Monico A, Berneman ZN, Franchini G. Protein isoforms encoded by the pX region of human T-cell leukemia/lymphotropic virus type I. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8813–8817.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

September 15, 1992

Volume

89

Issue

18

Start / End Page

8813 / 8817

Location

United States

Related Subject Headings

  • RNA Splicing
  • Polymerase Chain Reaction
  • Open Reading Frames
  • Oligodeoxyribonucleotides
  • Molecular Sequence Data
  • Humans
  • Human T-lymphotropic virus 1
  • HTLV-I Infections
  • Genes, pX
  • Genes, Overlapping