Sequencing and analysis of genomic fragments from the NF1 locus.
Publication
, Journal Article
Martin-Gallardo, A; Marchuk, DA; Gocayne, J; Kerlavage, AR; McCombie, WR; Venter, JC; Collins, FS; Wallace, MR
Published in: DNA Seq
1992
The sequence of five non-contiguous genomic fragments encompassing 14.4 kilobases from the NF1 locus have been determined by fluorescence-based automated DNA sequence analysis. These fragments included one kilobase of the NF1 coding region, which resulted in the identification of the intron/exon boundaries of five exons. Based on these sequences, five new NF1 exon-PCR assays have been developed, that could be useful for detecting new NF1 mutations. The genomic sequences were analyzed for the presence of Alu repetitive elements and their classification is described. This analysis may provide some insight into the characterization of genetic rearrangements resulting in disruption of the NF1 gene.
Duke Scholars
Published In
DNA Seq
DOI
ISSN
1042-5179
Publication Date
1992
Volume
3
Issue
4
Start / End Page
237 / 243
Location
England
Related Subject Headings
- Sequence Analysis, DNA
- Repetitive Sequences, Nucleic Acid
- Neurofibromatosis 1
- Molecular Sequence Data
- Introns
- Humans
- Genetics & Heredity
- Genes, Neurofibromatosis 1
- Exons
- DNA Transposable Elements
Citation
APA
Chicago
ICMJE
MLA
NLM
Martin-Gallardo, A., Marchuk, D. A., Gocayne, J., Kerlavage, A. R., McCombie, W. R., Venter, J. C., … Wallace, M. R. (1992). Sequencing and analysis of genomic fragments from the NF1 locus. DNA Seq, 3(4), 237–243. https://doi.org/10.3109/10425179209034023
Martin-Gallardo, A., D. A. Marchuk, J. Gocayne, A. R. Kerlavage, W. R. McCombie, J. C. Venter, F. S. Collins, and M. R. Wallace. “Sequencing and analysis of genomic fragments from the NF1 locus.” DNA Seq 3, no. 4 (1992): 237–43. https://doi.org/10.3109/10425179209034023.
Martin-Gallardo A, Marchuk DA, Gocayne J, Kerlavage AR, McCombie WR, Venter JC, et al. Sequencing and analysis of genomic fragments from the NF1 locus. DNA Seq. 1992;3(4):237–43.
Martin-Gallardo, A., et al. “Sequencing and analysis of genomic fragments from the NF1 locus.” DNA Seq, vol. 3, no. 4, 1992, pp. 237–43. Pubmed, doi:10.3109/10425179209034023.
Martin-Gallardo A, Marchuk DA, Gocayne J, Kerlavage AR, McCombie WR, Venter JC, Collins FS, Wallace MR. Sequencing and analysis of genomic fragments from the NF1 locus. DNA Seq. 1992;3(4):237–243.
Published In
DNA Seq
DOI
ISSN
1042-5179
Publication Date
1992
Volume
3
Issue
4
Start / End Page
237 / 243
Location
England
Related Subject Headings
- Sequence Analysis, DNA
- Repetitive Sequences, Nucleic Acid
- Neurofibromatosis 1
- Molecular Sequence Data
- Introns
- Humans
- Genetics & Heredity
- Genes, Neurofibromatosis 1
- Exons
- DNA Transposable Elements