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A yeast artificial chromosome contig encompassing the type 1 neurofibromatosis gene.

Publication ,  Journal Article
Marchuk, DA; Tavakkol, R; Wallace, MR; Brownstein, BH; Taillon-Miller, P; Fong, CT; Legius, E; Andersen, LB; Glover, TW; Collins, FS
Published in: Genomics
July 1992
Published version (DOI) Link to item

The yeast artificial chromosome (YAC) system (Burke et al., 1987, Science 236: 806-812) allows the direct cloning of large regions of the genome. A YAC contig map of approximately 700 kb encompassing the region surrounding the type 1 neurofibromatosis (NF1) locus on 17q11.2 has been constructed. A single YAC containing the entire NF1 locus has been constructed by homologous recombination in yeast. In the process of contig construction a novel method of YAC end rescue has been developed by YAC circularization in yeast and plasmid rescue in bacteria. YACs containing homology to the NF1 region but mapping to another chromosome have also been discovered. Sequences of portions of the homologous locus indicate that this other locus is a nonprocessed pseudogene.

Duke Scholars

Douglas Alan Marchuk
Author Douglas Alan Marchuk Molecular Genetics and Microbiology

Published In

Genomics

DOI

10.1016/0888-7543(92)90140-n

ISSN

0888-7543

Publication Date

July 1992

Volume

13

Issue

3

Start / End Page

672 / 680

Location

United States

Related Subject Headings

  • Sequence Homology, Nucleic Acid
  • Restriction Mapping
  • Pseudogenes
  • Plasmids
  • Molecular Sequence Data
  • Humans
  • Genome, Human
  • Genetics & Heredity
  • Genes, Neurofibromatosis 1
  • Gene Library
 

Citation

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ICMJE
MLA
NLM
Marchuk, D. A., Tavakkol, R., Wallace, M. R., Brownstein, B. H., Taillon-Miller, P., Fong, C. T., … Collins, F. S. (1992). A yeast artificial chromosome contig encompassing the type 1 neurofibromatosis gene. Genomics, 13(3), 672–680. https://doi.org/10.1016/0888-7543(92)90140-n
Marchuk, D. A., R. Tavakkol, M. R. Wallace, B. H. Brownstein, P. Taillon-Miller, C. T. Fong, E. Legius, L. B. Andersen, T. W. Glover, and F. S. Collins. “A yeast artificial chromosome contig encompassing the type 1 neurofibromatosis gene.” Genomics 13, no. 3 (July 1992): 672–80. https://doi.org/10.1016/0888-7543(92)90140-n.
Marchuk DA, Tavakkol R, Wallace MR, Brownstein BH, Taillon-Miller P, Fong CT, et al. A yeast artificial chromosome contig encompassing the type 1 neurofibromatosis gene. Genomics. 1992 Jul;13(3):672–80.
Marchuk, D. A., et al. “A yeast artificial chromosome contig encompassing the type 1 neurofibromatosis gene.” Genomics, vol. 13, no. 3, July 1992, pp. 672–80. Pubmed, doi:10.1016/0888-7543(92)90140-n.
Marchuk DA, Tavakkol R, Wallace MR, Brownstein BH, Taillon-Miller P, Fong CT, Legius E, Andersen LB, Glover TW, Collins FS. A yeast artificial chromosome contig encompassing the type 1 neurofibromatosis gene. Genomics. 1992 Jul;13(3):672–680.
Journal cover image

Published In

Genomics

DOI

10.1016/0888-7543(92)90140-n

ISSN

0888-7543

Publication Date

July 1992

Volume

13

Issue

3

Start / End Page

672 / 680

Location

United States

Related Subject Headings

  • Sequence Homology, Nucleic Acid
  • Restriction Mapping
  • Pseudogenes
  • Plasmids
  • Molecular Sequence Data
  • Humans
  • Genome, Human
  • Genetics & Heredity
  • Genes, Neurofibromatosis 1
  • Gene Library