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Cloning and expression of full-length cDNA encoding human vitamin D receptor.

Publication ,  Journal Article
Baker, AR; McDonnell, DP; Hughes, M; Crisp, TM; Mangelsdorf, DJ; Haussler, MR; Pike, JW; Shine, J; O'Malley, BW
Published in: Proc Natl Acad Sci U S A
May 1988
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Complementary DNA clones encoding the human vitamin D receptor have been isolated from human intestine and T47D cell cDNA libraries. The nucleotide sequence of the 4605-base pair (bp) cDNA includes a noncoding leader sequence of 115 bp, a 1281-bp open reading frame, and 3209 bp of 3' noncoding sequence. Two polyadenylylation signals, AATAAA, are present 25 and 70 bp upstream of the poly(A) tail, respectively. RNA blot hybridization indicates a single mRNA species of approximately equal to 4600 bp. Transfection of the cloned sequences into COS-1 cells results in the production of a single receptor species indistinguishable from the native receptor. Sequence comparisons demonstrate that the vitamin D receptor belongs to the steroid-receptor gene family and is closest in size and sequence to another member of this family, the thyroid hormone receptor.

Duke Scholars

Donald Patrick McDonnell
Author Donald Patrick McDonnell Pharmacology & Cancer Biology
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Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.85.10.3294

ISSN

0027-8424

Publication Date

May 1988

Volume

85

Issue

10

Start / End Page

3294 / 3298

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Recombinant Proteins
  • Receptors, Steroid
  • Receptors, Calcitriol
  • Nucleic Acid Hybridization
  • Molecular Sequence Data
  • Kinetics
  • Humans
  • Genes
  • DNA Restriction Enzymes
 

Citation

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Baker, A. R., McDonnell, D. P., Hughes, M., Crisp, T. M., Mangelsdorf, D. J., Haussler, M. R., … O’Malley, B. W. (1988). Cloning and expression of full-length cDNA encoding human vitamin D receptor. Proc Natl Acad Sci U S A, 85(10), 3294–3298. https://doi.org/10.1073/pnas.85.10.3294
Baker, A. R., D. P. McDonnell, M. Hughes, T. M. Crisp, D. J. Mangelsdorf, M. R. Haussler, J. W. Pike, J. Shine, and B. W. O’Malley. “Cloning and expression of full-length cDNA encoding human vitamin D receptor.” Proc Natl Acad Sci U S A 85, no. 10 (May 1988): 3294–98. https://doi.org/10.1073/pnas.85.10.3294.
Baker AR, McDonnell DP, Hughes M, Crisp TM, Mangelsdorf DJ, Haussler MR, et al. Cloning and expression of full-length cDNA encoding human vitamin D receptor. Proc Natl Acad Sci U S A. 1988 May;85(10):3294–8.
Baker, A. R., et al. “Cloning and expression of full-length cDNA encoding human vitamin D receptor.” Proc Natl Acad Sci U S A, vol. 85, no. 10, May 1988, pp. 3294–98. Pubmed, doi:10.1073/pnas.85.10.3294.
Baker AR, McDonnell DP, Hughes M, Crisp TM, Mangelsdorf DJ, Haussler MR, Pike JW, Shine J, O’Malley BW. Cloning and expression of full-length cDNA encoding human vitamin D receptor. Proc Natl Acad Sci U S A. 1988 May;85(10):3294–3298.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.85.10.3294

ISSN

0027-8424

Publication Date

May 1988

Volume

85

Issue

10

Start / End Page

3294 / 3298

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Recombinant Proteins
  • Receptors, Steroid
  • Receptors, Calcitriol
  • Nucleic Acid Hybridization
  • Molecular Sequence Data
  • Kinetics
  • Humans
  • Genes
  • DNA Restriction Enzymes