Isolation and characterization of the Escherichia coli mutL gene product.
Publication
, Journal Article
Grilley, M; Welsh, KM; Su, SS; Modrich, P
Published in: J Biol Chem
January 15, 1989
The Escherichia coli mutL gene product has been purified to near homogeneity from an overproducing clone. The mutL locus encodes a polypeptide of 70,000 daltons as determined by denaturing gel electrophoresis. The native molecular weight of MutL protein as calculated from the sedimentation coefficient of 5.5 S and Stokes radius of 61 A is 139,000 daltons, indicating that MutL exists as a dimer in solution. In addition to its ability to complement methyl-directed DNA mismatch repair in mutL-deficient cell-free extracts, DNase I protection experiments demonstrate that the purified MutL protein interacts with the MutS-heteroduplex DNA complex in the presence of ATP.
Duke Scholars
Published In
J Biol Chem
ISSN
0021-9258
Publication Date
January 15, 1989
Volume
264
Issue
2
Start / End Page
1000 / 1004
Location
United States
Related Subject Headings
- Molecular Weight
- Indicators and Reagents
- Hydroxyapatites
- Genes, Bacterial
- Genes
- Escherichia coli
- Electrophoresis, Polyacrylamide Gel
- Durapatite
- DNA Repair
- Chromatography, Gel
Citation
APA
Chicago
ICMJE
MLA
NLM
Grilley, M., Welsh, K. M., Su, S. S., & Modrich, P. (1989). Isolation and characterization of the Escherichia coli mutL gene product. J Biol Chem, 264(2), 1000–1004.
Grilley, M., K. M. Welsh, S. S. Su, and P. Modrich. “Isolation and characterization of the Escherichia coli mutL gene product.” J Biol Chem 264, no. 2 (January 15, 1989): 1000–1004.
Grilley M, Welsh KM, Su SS, Modrich P. Isolation and characterization of the Escherichia coli mutL gene product. J Biol Chem. 1989 Jan 15;264(2):1000–4.
Grilley, M., et al. “Isolation and characterization of the Escherichia coli mutL gene product.” J Biol Chem, vol. 264, no. 2, Jan. 1989, pp. 1000–04.
Grilley M, Welsh KM, Su SS, Modrich P. Isolation and characterization of the Escherichia coli mutL gene product. J Biol Chem. 1989 Jan 15;264(2):1000–1004.
Published In
J Biol Chem
ISSN
0021-9258
Publication Date
January 15, 1989
Volume
264
Issue
2
Start / End Page
1000 / 1004
Location
United States
Related Subject Headings
- Molecular Weight
- Indicators and Reagents
- Hydroxyapatites
- Genes, Bacterial
- Genes
- Escherichia coli
- Electrophoresis, Polyacrylamide Gel
- Durapatite
- DNA Repair
- Chromatography, Gel