DNA mismatch correction in a defined system.
Publication
, Journal Article
Lahue, RS; Au, KG; Modrich, P
Published in: Science
July 14, 1989
DNA mismatch correction is a strand-specific process involving recognition of noncomplementary Watson-Crick nucleotide pairs and participation of widely separated DNA sites. The Escherichia coli methyl-directed reaction has been reconstituted in a purified system consisting of MutH, MutL, and MutS proteins, DNA helicase II, single-strand DNA binding protein, DNA polymerase III holoenzyme, exonuclease I, DNA ligase, along with ATP (adenosine triphosphate), and the four deoxynucleoside triphosphates. This set of proteins can process seven of the eight base-base mismatches in a strand-specific reaction that is directed by the state of methylation of a single d(GATC) sequence located 1 kilobase from the mispair.
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Published In
Science
DOI
ISSN
0036-8075
Publication Date
July 14, 1989
Volume
245
Issue
4914
Start / End Page
160 / 164
Location
United States
Related Subject Headings
- Mutation
- Methylation
- General Science & Technology
- Escherichia coli
- DNA, Bacterial
- DNA Repair
- Base Sequence
Citation
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Lahue, R. S., Au, K. G., & Modrich, P. (1989). DNA mismatch correction in a defined system. Science, 245(4914), 160–164. https://doi.org/10.1126/science.2665076
Lahue, R. S., K. G. Au, and P. Modrich. “DNA mismatch correction in a defined system.” Science 245, no. 4914 (July 14, 1989): 160–64. https://doi.org/10.1126/science.2665076.
Lahue RS, Au KG, Modrich P. DNA mismatch correction in a defined system. Science. 1989 Jul 14;245(4914):160–4.
Lahue, R. S., et al. “DNA mismatch correction in a defined system.” Science, vol. 245, no. 4914, July 1989, pp. 160–64. Pubmed, doi:10.1126/science.2665076.
Lahue RS, Au KG, Modrich P. DNA mismatch correction in a defined system. Science. 1989 Jul 14;245(4914):160–164.
Published In
Science
DOI
ISSN
0036-8075
Publication Date
July 14, 1989
Volume
245
Issue
4914
Start / End Page
160 / 164
Location
United States
Related Subject Headings
- Mutation
- Methylation
- General Science & Technology
- Escherichia coli
- DNA, Bacterial
- DNA Repair
- Base Sequence