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Comparative immunogenicity in rhesus monkeys of multi-protein HIV-1 (CRF02_AG) DNA/MVA vaccines expressing mature and immature VLPs.

Publication ,  Journal Article
Ellenberger, D; Wyatt, L; Li, B; Buge, S; Lanier, N; Rodriguez, IV; Sariol, CA; Martinez, M; Monsour, M; Vogt, J; Smith, J; Otten, R; Moss, B ...
Published in: Virology
September 15, 2005

We developed an AIDS vaccine for Western and West-Central Africa founded on HIV-1 subtype CRF02_AG. Rhesus macaques were primed with Gag-Pol-Env-expressing plasmid DNA and boosted with a recombinant modified vaccinia virus Ankara (rMVA), expressing matched proteins. Two DNA vaccine constructs (IC1-90 and IC48) that differed by point mutations in gag and pol were compared. IC1-90 produces primarily immature (core comprises unprocessed Pr55Gag) HIV-like particles (VLPs) and IC48 produces mature VLP with processed Pr55Gag, immature VLP, and intracellular protein aggregates. Both vaccines raised significant cellular responses for Gag, Pol, and Env. Approximate twofold higher ELISPOT responses to Gag and Env epitopes were observed for IC48 animals than for IC1-90 animals at the peak post-MVA effector (P = 0.028) and late memory (P = 0.051) phases, respectively. Greater breadth for IC48-primed animals was observed than for IC1-90-primed animals at peak response (P = 0.03). Our results indicated that the vaccines elicited high frequency T cell responses and primed anti-Env antibody. They also suggest that expression of different forms of VLP has a significant effect on elicited cellular and humoral immunity.

Duke Scholars

Published In

Virology

DOI

ISSN

0042-6822

Publication Date

September 15, 2005

Volume

340

Issue

1

Start / End Page

21 / 32

Location

United States

Related Subject Headings

  • Virology
  • Vaccines, DNA
  • Transfection
  • T-Lymphocytes
  • Plasmids
  • Male
  • Macaca mulatta
  • Kidney
  • Humans
  • HIV-1
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Ellenberger, D., Wyatt, L., Li, B., Buge, S., Lanier, N., Rodriguez, I. V., … Butera, S. (2005). Comparative immunogenicity in rhesus monkeys of multi-protein HIV-1 (CRF02_AG) DNA/MVA vaccines expressing mature and immature VLPs. Virology, 340(1), 21–32. https://doi.org/10.1016/j.virol.2005.06.014
Ellenberger, Dennis, Linda Wyatt, Bin Li, Suzan Buge, Nattawan Lanier, I Vanesssa Rodriguez, Carlos A. Sariol, et al. “Comparative immunogenicity in rhesus monkeys of multi-protein HIV-1 (CRF02_AG) DNA/MVA vaccines expressing mature and immature VLPs.Virology 340, no. 1 (September 15, 2005): 21–32. https://doi.org/10.1016/j.virol.2005.06.014.
Ellenberger D, Wyatt L, Li B, Buge S, Lanier N, Rodriguez IV, et al. Comparative immunogenicity in rhesus monkeys of multi-protein HIV-1 (CRF02_AG) DNA/MVA vaccines expressing mature and immature VLPs. Virology. 2005 Sep 15;340(1):21–32.
Ellenberger, Dennis, et al. “Comparative immunogenicity in rhesus monkeys of multi-protein HIV-1 (CRF02_AG) DNA/MVA vaccines expressing mature and immature VLPs.Virology, vol. 340, no. 1, Sept. 2005, pp. 21–32. Pubmed, doi:10.1016/j.virol.2005.06.014.
Ellenberger D, Wyatt L, Li B, Buge S, Lanier N, Rodriguez IV, Sariol CA, Martinez M, Monsour M, Vogt J, Smith J, Otten R, Montefiori D, Kraiselburd E, Moss B, Robinson H, McNicholl J, Butera S. Comparative immunogenicity in rhesus monkeys of multi-protein HIV-1 (CRF02_AG) DNA/MVA vaccines expressing mature and immature VLPs. Virology. 2005 Sep 15;340(1):21–32.
Journal cover image

Published In

Virology

DOI

ISSN

0042-6822

Publication Date

September 15, 2005

Volume

340

Issue

1

Start / End Page

21 / 32

Location

United States

Related Subject Headings

  • Virology
  • Vaccines, DNA
  • Transfection
  • T-Lymphocytes
  • Plasmids
  • Male
  • Macaca mulatta
  • Kidney
  • Humans
  • HIV-1