Skip to main content
Journal cover image

Moderate hypothermia induces marked increase in levels and nuclear accumulation of SUMO2/3-conjugated proteins in neurons.

Publication ,  Journal Article
Wang, L; Ma, Q; Yang, W; Mackensen, GB; Paschen, W
Published in: J Neurochem
November 2012

Deep hypothermia protects the brain from ischemic damage and is therefore used during major cardiovascular surgeries requiring cardiopulmonary bypass and a period of circulatory arrest. Here, we demonstrated that small ubiquitin-like modifier (SUMO1-3) conjugation is markedly activated in the brain during deep to moderate hypothermia. Animals were subjected to normothermic (37°C) or deep to moderate (18°C, 24°C, 30°C) hypothermic cardiopulmonary bypass, and the effects of hypothermia on SUMO conjugation were evaluated by Western blot and immunohistochemistry. Exposure to moderate 30°C hypothermia was sufficient to markedly increase levels and nuclear accumulation of SUMO2/3-conjugated proteins in these cells. Deep hypothermia induced nuclear translocation of the SUMO-conjugating enzyme Ubc9, suggesting that the increase in nuclear levels of SUMO2/3-conjugated proteins observed in brains of hypothermic animals is an active process. Exposure of primary neuronal cultures to deep hypothermia induced only a moderate rise in levels of SUMO2/3-conjugated proteins. This suggests that neurons in vivo have a higher capacity than neurons in vitro to activate this endogenous potentially neuroprotective pathway upon exposure to hypothermia. Identifying proteins that are SUMO2/3 conjugated during hypothermia could help to design new strategies for preventive and therapeutic interventions to make neurons more resistant to a transient interruption of blood supply.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Neurochem

DOI

EISSN

1471-4159

Publication Date

November 2012

Volume

123

Issue

3

Start / End Page

349 / 359

Location

England

Related Subject Headings

  • Ubiquitin-Conjugating Enzymes
  • Subcellular Fractions
  • Small Ubiquitin-Related Modifier Proteins
  • Rats, Sprague-Dawley
  • Rats
  • Primary Cell Culture
  • Nuclear Proteins
  • Neurons
  • Neurology & Neurosurgery
  • Male
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wang, L., Ma, Q., Yang, W., Mackensen, G. B., & Paschen, W. (2012). Moderate hypothermia induces marked increase in levels and nuclear accumulation of SUMO2/3-conjugated proteins in neurons. J Neurochem, 123(3), 349–359. https://doi.org/10.1111/j.1471-4159.2012.07916.x
Wang, Liangli, Qing Ma, Wei Yang, G Burkhard Mackensen, and Wulf Paschen. “Moderate hypothermia induces marked increase in levels and nuclear accumulation of SUMO2/3-conjugated proteins in neurons.J Neurochem 123, no. 3 (November 2012): 349–59. https://doi.org/10.1111/j.1471-4159.2012.07916.x.
Wang L, Ma Q, Yang W, Mackensen GB, Paschen W. Moderate hypothermia induces marked increase in levels and nuclear accumulation of SUMO2/3-conjugated proteins in neurons. J Neurochem. 2012 Nov;123(3):349–59.
Wang, Liangli, et al. “Moderate hypothermia induces marked increase in levels and nuclear accumulation of SUMO2/3-conjugated proteins in neurons.J Neurochem, vol. 123, no. 3, Nov. 2012, pp. 349–59. Pubmed, doi:10.1111/j.1471-4159.2012.07916.x.
Wang L, Ma Q, Yang W, Mackensen GB, Paschen W. Moderate hypothermia induces marked increase in levels and nuclear accumulation of SUMO2/3-conjugated proteins in neurons. J Neurochem. 2012 Nov;123(3):349–359.
Journal cover image

Published In

J Neurochem

DOI

EISSN

1471-4159

Publication Date

November 2012

Volume

123

Issue

3

Start / End Page

349 / 359

Location

England

Related Subject Headings

  • Ubiquitin-Conjugating Enzymes
  • Subcellular Fractions
  • Small Ubiquitin-Related Modifier Proteins
  • Rats, Sprague-Dawley
  • Rats
  • Primary Cell Culture
  • Nuclear Proteins
  • Neurons
  • Neurology & Neurosurgery
  • Male