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Sedimentation properties of yeast chromosomal DNA.

Publication ,  Journal Article
Petes, TD; Fangman, WL
Published in: Proc Natl Acad Sci U S A
May 1972

Sedimentation analysis of nuclear DNA released from spheroplasts of the yeast Saccharomyces cerevisiae indicates that it has a number average molecular weight of 6.2 x 10(8). The chromosomal DNA molecules range in size from as small as 5 x 10(7) daltons to as large as 1.4 x 10(9) daltons. Based on these values and estimates of the total DNA content of the yeast nucleus, it is proposed that each yeast chromosome contains a single DNA duplex.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

May 1972

Volume

69

Issue

5

Start / End Page

1188 / 1191

Location

United States

Related Subject Headings

  • Uracil
  • Tritium
  • Saccharomyces cerevisiae
  • Saccharomyces
  • Molecular Weight
  • DNA, Viral
  • DNA
  • Coliphages
  • Chromosomes
  • Centrifugation, Density Gradient
 

Citation

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Petes, T. D., & Fangman, W. L. (1972). Sedimentation properties of yeast chromosomal DNA. Proc Natl Acad Sci U S A, 69(5), 1188–1191. https://doi.org/10.1073/pnas.69.5.1188
Petes, T. D., and W. L. Fangman. “Sedimentation properties of yeast chromosomal DNA.Proc Natl Acad Sci U S A 69, no. 5 (May 1972): 1188–91. https://doi.org/10.1073/pnas.69.5.1188.
Petes TD, Fangman WL. Sedimentation properties of yeast chromosomal DNA. Proc Natl Acad Sci U S A. 1972 May;69(5):1188–91.
Petes, T. D., and W. L. Fangman. “Sedimentation properties of yeast chromosomal DNA.Proc Natl Acad Sci U S A, vol. 69, no. 5, May 1972, pp. 1188–91. Pubmed, doi:10.1073/pnas.69.5.1188.
Petes TD, Fangman WL. Sedimentation properties of yeast chromosomal DNA. Proc Natl Acad Sci U S A. 1972 May;69(5):1188–1191.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

May 1972

Volume

69

Issue

5

Start / End Page

1188 / 1191

Location

United States

Related Subject Headings

  • Uracil
  • Tritium
  • Saccharomyces cerevisiae
  • Saccharomyces
  • Molecular Weight
  • DNA, Viral
  • DNA
  • Coliphages
  • Chromosomes
  • Centrifugation, Density Gradient