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Cholesterol homeostasis in the rat with a portacaval anastomosis.

Publication ,  Journal Article
Proia, A; McNamara, DJ; Edwards, KD; Ahrens, EH
Published in: Proc Natl Acad Sci U S A
September 1979

Studies were undertaken to determine the effect of portacaval anastomosis on cholesterol homeostasis in rats fed sucrose/lard under conditions of normal body growth. Four to 6 weeks after portacaval shunt surgery, we found decreases in plasma cholesterol and triglyceride concentrations, total liver weight, and hepatic microsomal protein concentration. Measurements oof hepatic 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase (EC 1.1.1.34) activity showed decreases in specific activity and total liver activity in portacaval shunt rats, but the enzyme diurnal rhythm remained. Decreased reductase activity in shunted rats was not due to an altered Km for D-HMG-COA, nor was an enzyme inhibitor found in the livers of the portacaval shunt animals. Sterol balance measurements in rats with shunts showed a 22% decrease in whole body cholesterol synthesis rate compared to controls. These metabolic studies, coupled with postmortem data, showed diminished bile acid synthesis, unchanged fecal neutral steroid excretion, and decreased net tissue accumulation of cholesterol during growth. The decreased whole body cholesterol synthesis rate ultimately led to a diminished total carcass cholesterol concentration in the rats with shunts.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

September 1979

Volume

76

Issue

9

Start / End Page

4654 / 4657

Location

United States

Related Subject Headings

  • Rats
  • Portacaval Shunt, Surgical
  • Liver
  • Kinetics
  • Hyperlipoproteinemia Type II
  • Hydroxymethylglutaryl CoA Reductases
  • Homeostasis
  • Growth
  • Dietary Carbohydrates
  • Cholesterol
 

Citation

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Proia, A., McNamara, D. J., Edwards, K. D., & Ahrens, E. H. (1979). Cholesterol homeostasis in the rat with a portacaval anastomosis. Proc Natl Acad Sci U S A, 76(9), 4654–4657. https://doi.org/10.1073/pnas.76.9.4654
Proia, A., D. J. McNamara, K. D. Edwards, and E. H. Ahrens. “Cholesterol homeostasis in the rat with a portacaval anastomosis.Proc Natl Acad Sci U S A 76, no. 9 (September 1979): 4654–57. https://doi.org/10.1073/pnas.76.9.4654.
Proia A, McNamara DJ, Edwards KD, Ahrens EH. Cholesterol homeostasis in the rat with a portacaval anastomosis. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4654–7.
Proia, A., et al. “Cholesterol homeostasis in the rat with a portacaval anastomosis.Proc Natl Acad Sci U S A, vol. 76, no. 9, Sept. 1979, pp. 4654–57. Pubmed, doi:10.1073/pnas.76.9.4654.
Proia A, McNamara DJ, Edwards KD, Ahrens EH. Cholesterol homeostasis in the rat with a portacaval anastomosis. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4654–4657.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

September 1979

Volume

76

Issue

9

Start / End Page

4654 / 4657

Location

United States

Related Subject Headings

  • Rats
  • Portacaval Shunt, Surgical
  • Liver
  • Kinetics
  • Hyperlipoproteinemia Type II
  • Hydroxymethylglutaryl CoA Reductases
  • Homeostasis
  • Growth
  • Dietary Carbohydrates
  • Cholesterol